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Fig. 3 | EvoDevo

Fig. 3

From: Whole body regeneration and developmental competition in two botryllid ascidians

Fig. 3

Secondary bud migration and development following removal of zooids and other developing buds. A Post-surgery darkfield image showing vasculature (demarcated by white dashed line), tunic, and a single remaining stage D secondary bud (outlined in red dashed line). B After 24 h, the secondary bud is adjacent to the vasculature. C, D At 2- and 3-days post-surgery, the secondary bud continued development and had merged with the vasculature. Subsequent panels indicate location of secondary bud (white arrows). E–I The secondary bud shows pigmentation and is now evident under the microscope. The heartbeat developed during this 5–7-day time frame which allowed for easy detection. J Ten days post-surgery an open siphon is visible (black arrow) and the zooid was actively feeding. dps days post-surgery. Scale bars = 250 µm

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