Fig. 8
From: Whole body regeneration and developmental competition in two botryllid ascidians
Loser bud resorption increased size of winner bud. A Illustration showing surgery performed to isolate secondary bud (white arrow). B, C Post-surgery darkfield images of secondary bud (white dashed circle) at day 0 and 9, respectively. D Higher magnification of zooid in C at time of siphon opening. E Size of a control zooid upon siphon opening. F Illustration showing surgery performed to isolate two secondary buds (white arrows). G, H Post-surgery darkfield images showing secondary buds (white circles) at day 0 and 7, respectively. I Higher magnification of C zooid at time of siphon development. J Quantitative analysis comparing areas of isolated secondary bud(s) vs. normal/blastogenic bud. Heartbeat and siphon opening used as developmental landmarks. dps days post-surgery. Asterisks indicate statistical significance (*P < 0.05). n.s. = not significant. Scale bars = 0.5 mm