Fig. 9

Secondary bud resorption is reversed after blood-borne crosstalk severed. A Post-surgery darkfield image of vasculature with two secondary buds (white dashed circles) connected through the blood vasculature. B At 4 days post-surgery, both secondary buds had fused to the common vasculature and continued development. C On day 8, the left secondary bud was identified as the purported winner because the right secondary bud had ceased growth. After image was taken, a section of blood vessels between secondary buds was removed. D By day 12 the loser bud had opened a siphon and began asexually budding. dps days post-surgery. Scale bars = 1.0 mm