Fig. 3

Immunostainings of Distal-less and Spalt proteins in other nymphalids with spot and eyespot patterns. In all species surveyed here, both Dll and Sal proteins are present in spots and eyespot patterns in late fifth instar larval and 24–28 h pupal wings. Note that both proteins are also expressed in wing patterns that map to parafocal, submarginal and marginal pattern systems as outlined in the nymphalid ground plan. Scale bars for (K and L—1500 µm); (C and D—1000 µm); (A, B, E, F, G, H, I, and J—500 µm); (Gʹ and Hʹ—200 µm); (Aʹ, Bʹ, Cʹ, Dʹ, Eʹ, Fʹ, Iʹ, Jʹ, Kʹ and Lʹ—50 µm). The expression of Sal on the right in panel Kʹ corresponds to another, more posterior eyespot.