Fig. 5

Distal-less functions in the development of wing margin melanic scale development in P. canidia. A Structure of the Distal-less locus and location of the two sgRNAs used to disrupt the locus in exons 2 (E2) and exon 3 (E3) (red pins). B Dll crispants had indels in both E2 and E3 that were detected using Next-Generation sequencing. C Various Dll crispants generated through CRISPR/Cas9 of both E2 and E3. Phenotypes include disrupted scale development and possible loss of melanism as supported by aberrant phenotypes obtained in D defective wing margin with loss of both black and white scales within the affected area, F loss of black and white scales in the wing apex, and E, G transformation of black scales in chevron areas to white scales. D–G Close-up of the mosaic area affected by the CRISPR knock-out experiments. Crispants shown here were affected by disruptions in both Exons 2 and 3