Fig. 6
From: Distal-less and spalt are distal organisers of pierid wing patterns
spalt functions in black scale development in P. canidia. A Structure of the spalt locus and area targeted by the sgRNA (red pin). B Spalt crispants had indels in the target region that were detected using Sanger sequencing. C Various spalt crispants (mosaic mutants) generated through CRISPR/Cas9. Phenotypes include missing spots or missing black scales in spots, disrupted Cu2 veins, missing black chevrons located along the wing margin (M8), and less melanised spots (M9). D–F Close-up of mosaic areas affected. G Close-up of black spot pattern in wild-type P. canidia.