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Fig. 4 | EvoDevo

Fig. 4

From: Characterizing Hox genes in mayflies (Ephemeroptera), with Hexagenia limbata as a new mayfly model

Fig. 4

Horseradish peroxidase staining of H. limbata embryos using the Antp (4C3) antibody (a, b, d, fm), with select corresponding DAPI stains (c, e). Early embryos do not show any expression (a). As the embryo adds segments to the posterior, expression is present in the three thoracic segments, where thoracic limb buds will eventually protrude (b, arrows in c and magnified views in d, e). As the limb buds elongate, expression extends into the buds (f, g), eventually appearing as patches of strong expression at the anterior of T2 and T3 (arrowheads in h, magnified view in i), while expression at the midline of each segment is less strong (h, i). At this time, expression also begins to faintly appear on the posterior edge of the labial segment (h, arrow in i). After segmentation concludes, strong expression in the thorax and thoracic limbs is present, weak expression is still evident at the posterior edge of the labial segment, and expression is seen in the center of the A1–A3 segments (j, k). Thoracic limb expression is strongest in the proximal region and grows fainter at the distal tips (arrows in l). In the oldest embryos we imaged, expression in the abdomen becomes stronger and extends up to the A10 segment, but is absent from the lateral edges (m). Expression at this stage extends from the posterior of the labial segment through the A10 segment (m). Mn, mandible; Mx, maxilla; Lb, labium; T1–T3, thoracic segments; A1–A10, abdominal segments. Ventral view, anterior to the top in all panels. Scale bars 0.10 mm. Image magnifications are 200X for a, d, e, and jm, 100X for b, c and fi

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