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Table 1 Assembly and quality metrics for ORP and CLC transcriptomes of H. limbata reads

From: Characterizing Hox genes in mayflies (Ephemeroptera), with Hexagenia limbata as a new mayfly model

Criteria

ORP

CLC

Number of contigs

83,795

93,561

Number of contigs with ORFs

15,543

16,100

Number of annotated contigs

25,134

NA*

Mean contig length

702

529.36

Longest contig

26,091

22,280

Shortest contig

201

82

TransRate Assembly score

0.215

0.022

TransRate Optimal score

0.243

0.035

BUSCO Complete Score

1213 (88.8%)

839 (61.4%)

  1. The number of contigs with ORFs was provided by TransRate. The TransRate assembly score assesses how accurate and complete an assembly is, while the optimal score is the assembly score obtained after removing all poorly assembled contigs. The complete BUSCO score represents the number of database sequences identified in the assembly, and was calculated using the insect ortholog database Odb10
  2. *The number of annotated ORFs was determined via Diamond annotation and was not assessed for CLC contigs
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