Fig. 1From: Adenylate cyclase A amplification and functional diversification during Polyspondylium pallidum developmentExpression patterns of P. pallidum aca genes. Ppal wild-type cells were transformed with gene fusions of the LacZ reporter and the intergenic regions upstream of the start-codon of the Ppal aca1, aca2 and aca3 genes. The cells were developed on NN agar until streaming aggregates (top), primary sorogens (centre) and secondary sorogens (bottom) had formed and intact structures were then fixed with glutaraldehyde and stained with X-gal. Bars: 0.1Â mmBack to article page