Fig. 3
Development of aca3ˉaca2ˉ and aca1ˉaca3ˉaca2ˉ mutants. A Aggregation: Ppal aca3ˉaca2ˉ and aca1ˉaca3ˉaca2ˉ were starved on NN agar at 106 cells/cm2 on their own or mixed with 10% wild-type Ppal for the indicated time periods. Bar: 1 mm. B Fruiting bodies: the cells were developed into fruiting bodies, which were imaged in situ. Bars: 0.5 mm. C Spore and stalk cells: Ppal WT and aca1ˉaca3ˉaca2ˉ fruiting bodies were transferred to 0.001% Calcofluor and imaged under phase contrast (left) and epifluorescence (right). Bar: 20 µm