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Fig. 5 | EvoDevo

Fig. 5

From: Adenylate cyclase A amplification and functional diversification during Polyspondylium pallidum development

Fig. 5

cAMP relay in Ppal wild-type and Ddis acaˉ/ACG. A P. pallidum. Ppal WT was starved on agar for 4 h or until streaming aggregates, tipped mounds and aerially lifted sorogens had formed. Structures were gently dissociated, resuspended in PB to 108 cells/ml and stimulated at t = 0 min with 10 μM 2'H-cAMP and 5 mM DTT. Reactions were terminated with 1.75% perchloric acid at the indicated timepoints and cAMP was assayed by isotope dilution assay. B D. discoideum Vegetative WT AX3 and acaˉ/ACG cells, and cells starved on NN agar for 4 h were resuspended in PB to 108 cells/ml and stimulated with 5 μM 2'H-cAMP and 5 mM DTT in the presence and absence of 1 mM IPA. Reactions were terminated as above, and cAMP was assayed. Data were standardized to the protein content of the cell suspensions and represent means and SE of six experiments performed in triplicate for Ppal sorogens and two experiments in triplicate for other stages and cell lines. The experiments in panel B were performed twice more in triplicate with acaˉ/ACG cells in the absence of IPA, with similar results

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