Fig. 2

Upregulated germline-related OrthoGroups (OGs) shared by 8 species or more. A The table represents presence (light blue) or absence (light grey) in different species (columns) of germline-related differentially transcribed genes belonging to different OGs (rows). On the right of each row is reported the annotation of proteins encoded by genes included in the respective OGs: bold names represent proteins associated to DNA-related activities; Sodium-dependent transporters is included between parentheses because it represents a protein family, since the corresponding OG was a large cluster of homology, and not a defined orthology group. On the right, a table summarizes the gene nomenclature in three model species (Hsa: Homo sapiens; Cel: C. elegans; Dme: D. melanogaster). Asterisks on the left are associated to those genes whose transcription was upregulated in germline-related samples in 8 or more species also with other more stringent logFC cut-offs and DESeq2/edgeR intersection (see “Methods”). B The lower table represents absence/presence in the species (columns refers to upper table A) of transcripts of genes commonly associated to GMP (row names are gene products). Excluding Piwi (present also in the upper table A), only Nanos, Vasa, and Tudor were upregulated in a conspicuous number of species in our data set (see Additional file 2: Fig. S1 for GMP domain enrichment)