Fig. 3

Zygotic expression onset of tissue-specific effector genes during the development of H. pulcherrimus. A Indicates the number of tissue-specific effector genes calculated as expressed in a specific number of cell lineage(s). B and C Show the number of tissue-specific effector genes with zygotic expression onset at each timepoint. The color of each bar in A–C reflects the number of cell lineages in which tissue-specific effector genes were expressed at the early gastrula stage. B Shows the number of genes at each developmental time stacked together, and C shows the number of genes expressed in each number of cell lineages separately. D Shows the number of tissue-specific effector genes whose expression was restricted to a single cell lineage at each zygotic expression onset time and each cell lineage (as shown in the bottom graphs in C). The number and cell lineages are reflected in the size and color of the circles, respectively. Bars 1–4 in D indicate the cell fate segregation timing with reference to Fig. 1. Bar 1: micromere to skeletogenic cells and germline; Bar 2: mesomere to apical ectoderm and macromere to Veg1/Veg2; Bar 3: nonapical ectoderm to ciliary ectoderm/nerve cells and Veg2 to Veg2 endoderm/NSM; Bar 4: Veg1 to Veg1 ectoderm/Veg1 endoderm