Understanding the basis of a novel fruit type in Brassicaceae: conservation and deviation in expression patterns of six genes
© Avino et al.; licensee BioMed Central Ltd. 2012
Received: 16 March 2012
Accepted: 28 June 2012
Published: 3 September 2012
Variation in fruit morphology is important for plant fitness because it influences dispersal capabilities. Approximately half the members of tribe Brassiceae (Brassicaceae) exhibit fruits with segmentation and variable dehiscence, called heteroarthrocarpy. The knowledge of the genetics of fruit patterning in Arabidopsis offers the opportunity to ask: (1) whether this genetic pathway is conserved in taxa with different fruit morphologies; (2) how the pathway may be modified to produce indehiscence; and (3) whether the pathway has been recruited for a novel abscission zone.
We identified homologs of ALCATRAZ, FRUITFULL, INDEHISCENT, SHATTERPROOF, and REPLUMLESS from two taxa, representing different types of heteroarthrocarpy. ComParative gene expression of twelve loci was assessed to address how their expression may have been modified to produce heteroarthrocarpy.
Studies demonstrated overall conservation in gene expression patterns between dehiscent segments of Erucaria erucarioides and Arabidopsis, with some difference in expression of genes that position the valve margin. In contrast, indehiscence in heteroarthrocarpic fruit segments was correlated with the elimination of the entire valve margin pathway in Erucaria and Cakile lanceolata as well as its absence from a novel lateral abscission zone.
These findings suggest that modifications in the valve margin positioning genes are responsible for differences between heteroarthrocarpic and Arabidopsis-like fruits and support the hypothesis that heteroarthrocarpy evolved via repositioning the valve margin. They also highlight conservation in the dehiscence pathway across Brassicaceae.
KeywordsALCATRAZ Brassicaceae Fruit development INDEHISCENT FRUITFULL REPLUMLESS SHATTERPROOF Silique
In evolutionary developmental genetic studies, flowers have largely overshadowed fruits despite the fact that fruits display the same diversity in form (reviewed in). Variation in fruit morphology is fundamentally tied to plant dispersal, a key component of angiosperm fitness. Not only have fleshy, dry dehiscent, and dry indehiscent fruits evolved multiple times across flowering plants[2, 3], differences in fruit morphology are also observed at close phylogenetic distances within families. For example, berries have evolved more than once in both Solanaceae and Melastomataceae. Even families that have unique fruit types such as Fabaceae (legume) or Brassicaceae (silique) display considerable variation in form, segmentation, shape, and dehiscence capabilities[6–9]. Here we investigate the basis of fruit diversity within the Brassicaceae, the family that houses the genetic model Arabidopsis thaliana.
The genetic basis of dry dehiscent fruits has been thoroughly investigated in Arabidopsis (reviewed in[10–12]), providing a valuable framework for comParative studies of other brassicaceous fruits. Arabidopsis has a typical silique (hereafter referred to as typical silique), which is a bicarpellate capsule with two valves that dehisce or open at maturity. The valves surround the entire ovary and eventually seParate from persistent placental tissue (replum) along the valve margin. The replum is connected by a thin membranous septum that seParates the ovary into two chambers. The valve margin comprises two layers: a thick line of cells adjacent to the replum that form the seParation layer, and a group of lignified cells adjacent to the valve that form the lignified layer. The valves detach through the enzymatic breakdown of the middle lamella of the cells in the seParation layer, aided by the mechanical tension of lignified cells in the lignified layer and in the endocarp b (endb) layer of the valve. Thus, fruit opening in Arabidopsis is dependent on the proper positioning and formation of the valve margin and its dehiscent zone (DZ).
Recent genetic studies on fruit development in Arabidopsis have emphasized the patterning and positioning of the valve margin. The valve margin and its subsequent DZ are determined by the combined activities of SHATTERPROOF1 (SHP1), SHATTERPROOF2 (SHP2), INDEHISCENT (IND), and ALCATRAZ (ALC), which represent a genetic pathway[11, 14]. The closely related MADS-box containing Paralogs SHP1/2 are both expressed in the ovules, septum, and valve margin[15, 16]. SHP1/2 are functionally redundant and their double mutant phenotype exhibits a loss of valve margin identity with the resultant loss of the DZ. IND and ALC, both encoding bHLH proteins, are genetically downstream of SHP1/2[11, 14]. IND is the more pleiotropic of the two, specifying the identity of both the seParation and lignified cell layers of the valve margin. ALC plays a more limited role in the differentiation only of the seParation layer. The MADS-box gene FRUITFULL (FUL) and the BEL1-class homeodomain gene REPLUMLESS (RPL) are required to restrict SHP1/2, ALC, and IND to the narrow strip of cells at the valve/replum boundary[14, 19, 20]. FUL is expressed in the valves whereas RPL is expressed in the replum. Thus, the two genes play complementary roles to restrict the development of the valve margin identity to a thin region of cells via the repression of the SHP1/2, IND, and ALC[14, 20]. RPL repression of SHP1/2 in the valve margin is likely mediated by limiting the expression of JAGGED (JAG), FILAMENTOUS FLOWER (FIL), and YABBY3 (YAB3), which in turn promote FUL and SHP1/SHP2 expression. Both FUL and RPL are valve margin positioning genes. Whereas FUL is not required to induce valve formation, evidence indicates that RPL contributes to replum formation[21–23]. Studies in Brassica, a member of Brassicaceae with fruits similar to Arabidopsis, demonstrate that the roles of FUL, IND, and RPL in this pathway have been conserved[23–25], however the entire pathway has not been examined in any indehiscent relative.
Heteroarthrocarpy has important implications for dispersal because indehiscence creates dispersal propagules that are protected by the pericarp, and the joint results in seeds from the same plant being passively dispersed in seParate and often morphologically different propagule types[32–34]. Moreover, variants of heteroarthrocarpy reflect differences in dehiscence capabilities. Here we focus on two closely related species, Erucaria erucarioides and Cakile lanceolata, that display different types of heteroarthrocarpy[28, 29]. The proximal segment of Erucaria shows valve dehiscence but the joint itself does not abscise (Figure1). As such, the seeds in the proximal segment are dispersed freely into the environment when the valves open, in essentially the same manner as observed in Arabidopsis, whereas seeds in the distal segment are dispersed in their enclosing pericarp often when the maternal plant dies. In contrast, Cakile has heteroarthrocarpic fruits that are completely indehiscent with a joint that abscises at maturation, seParating the fruit into two segments (Figure1). Although all the seeds in the fruit are dispersed enclosed in pericarp, the distal segment is freely dispersed when the joint abscises, while seeds contained in the proximal segment frequently remain attached to the maternal plant even after its death.
To understand the genetic basis of heteroarthrocarpy and variation in dehiscence, we isolated homologs of all major genes in the valve margin genetic pathway of Arabidopsis from Cakile and Erucaria and compared gene expression patterns throughout flower and fruit development. Examination of these taxa permits comparisons within fruits as well as between species. Furthermore, focusing on these two species allowed us to simultaneously explore the extent to which expression patterns in the genetic pathway of Arabidopsis have been conserved with regards to dehiscence as well as how they may be modified in species with indehiscent and segmented fruits. We expected to observe similar expression patterns in members of the genetic pathway between the dehiscent segment of Erucaria and Arabidopsis, due to the anatomical similarities. We also predicted the loss of expression of at least some of the valve margin pathway genes in the ovary wall of indehiscent segments. However, differences between the distal and proximal segments were expected because the proximal segment of Cakile is valvular in origin in contrast with the distal segments of both species. We had no a priori predictions where the pathway may be disrupted in indehiscent segments because dehiscence in Arabidopsis can be lost by loss of function mutations in all genes of the valve margin pathway as well as overexpression of FUL (reviewed in). We hypothesized that the valve margin pathway might have been recruited to pattern the novel dehiscence zone in the joint of Cakile because the joint is anatomically similar to the DZ between the valve and replum[29, 35]. Our data support conservation of gene expression patterns between dehiscent proximal segments of Erucaria and fruits of Arabidopsis, especially regarding the genes that promote valve margin identity. Notably, there was less conservation in the genes responsible for positioning the valve margin. Indehiscence was correlated with loss of gene expression of the entire valve margin genetic pathway in both the indehiscent, proximal segment of Cakile and the distal segments of both species. Surprisingly, the joint was also characterized by the absence of expression of valve margin genes. The implications of these patterns for the evolution of heteroarthrocarpy are discussed.
Plant material and growth conditions
Seeds of Cakile lanceolata (Willd.) O.E. Schulz and Erucaria erucarioides (Coss. and Durieu) MüllBerol were obtained from Russell Reardon (US Department of Commerce, National Oceanic and Atmospheric Administration, Florida Keys National Marine Sanctuary) and the late César Gómez-Campo’s seed collection, respectively. Species were grown from seed in the Harvard University greenhouses and the University of Alberta, Department of Biological Sciences growth chambers. Flowers of both focal taxa were hand pollinated to ensure seed set.
Identification of valve margin pathway homologs
RNA was extracted from developing flowers and fruits using Concert Plant RNA Reagent kit (Invitrogen, Carlsbad, CA, USA), DNAse1 treated to remove DNA (Fermentas, Honover, MD, USA) and then cleaned with RNAeasy MiniKit (Qiagen, MD, USA). mRNA was purified from total RNA with the Dynabeads mRNA Purification Kit (Invitrogen, Karlsruhe, Germany). cDNA was then synthesized from the mRNA using the RevertAid H Minus First Strand cDNA Synthesis Kit (Fermentas, St Leon-Rot, Germany) or the SuperScriptII reverse transcriptase (Invitrogen, Carlsbad, CA, USA) by priming with polyT primer. ALC-, FUL-, IND-, RPL-, and SHP1/2-like genes were amplified via PCR from Erucaria and Cakile cDNA. In order to establish copy number, we used a set of broadly degenerate primers that picked up other related loci and used multiple primer combinations (see Additional file1: Table S1, for primer information and sequences). Primers were designed using mostly a nested primer design and by comparison to known sequences, particularly within Arabidopsis thaliana. PCR products were cloned into TOPO-TA (Invitrogen, Carlsbad, CA, USA). An average of 42 independent clones were sequenced per identified locus with a minimum number of 10 sequenced. Restriction analysis was also employed to screen colonies for potentially different copies.
Sequences were manually edited in MacVector v. 11.1 (MacVector Inc., Cary, NC, USA) and putative gene identity was initially established with BLASTn searches. To confirm gene homology, phylogenetic analyses were then conducted. Amino acid sequences were aligned with GenBank sequences (Additional file2: Table S2) in either MacVector or BioEdit v.18.104.22.168. Neighbor-joining (NJ) trees were generated in MEGA v.5. As we are primarily interested in identifying homologs, not reconstructing phylogenies to infer relationships amongst genes, the NJ approach was sufficient to assess homology.
In situ hybridization
In situ hybridization is the best available method for obtaining gene expression patterns in space and time, which is especially important when examining genes that potentially exhibit within-organ patterning (for example, within the presumptive valve margin of the carpel). Moreover, the candidate loci show a very close correspondence between their expression patterns and their function in Arabidopsis, although this may not hold true for Cakile and Erucaria. Developing inflorescences (including floral meristems), flowers and fruits from Cakile and Erucaria were collected and fixed under vacuum in freshly prepared, cold FAA (50% ethanol, 4% formalin, and 5% glacial acetic acid). We were unable to conduct in situ hybridization on mature fruits, as their heavily lignified tissue is not amenable to sectioning. After an incubation of 12 to 16 h, specimens were dehydrated in an ethyl alcohol series, embedded in Paraplast, and stored at 4°C until use. Wax blocks were sectioned to 8μm on a Reichert-Jung (Buffalo, NY, USA) or a Microm HM 325 (Walldorf, Germany) microtome. DNA templates for RNA probe synthesis were obtained by PCR amplification of 150- to 500-bp fragments from cDNA clones of 12 loci: ClALC, ClFUL1, ClFUL2, ClRPL, ClSHP1, ClSHP2, EeALC, EeFUL1, EeFUL2, EeIND, EeRPL, and EeSHP2 (Additional file1: Table S1 for primer information). Probe templates included 100 to 250 bp of the 3′ untranslated region in addition to 50 to 250 bp from the 3′ coding region for gene specificity, except the EeSHP2 and ClSHP2 probes included only the 3′ coding region. Probes for recent Paralogs, SHP1/SHP2 and FUL1/FUL2, were designed in regions of highest variation between Paralogs. Fragments were cloned using the TOPO-TA plasmid vector (Invitrogen, Carlsbad, CA, USA) and confirmed by sequencing. Both sense and antisense digoxigenin-labeled RNA (Roche, Indianapolis, IN, USA) probes were prepared from linearized template plasmids and alkaline hydrolyzed to 150 to 300 bp. In situ hybridization was then performed following described methods. Sections were visualized and imaged using a combination of white and fluorescent light after counterstaining with calcofluor. Sections were digitally photographed using a Leica Leitz DMRD microscope equipped with a Retiga EXi imaging system (Harvard University imaging center) or a NIKON H550L fluorescence microscope equipped with a Nikon DS-Ri1 imaging system (University of Alberta).
Characterization of differential expression in floral organs of Cakile and Erucaria was performed using reverse transcription-polymerase chain reaction (RT‐PCR) on cDNA from the following tissue: (1) young buds (<4 mm), corresponding to carpels before differentiation into discernable replum, ovary wall/valve, and endocarp; (2) old buds (>4 mm), corresponding to carpels after differentiation; (3) flowers at anthesis; (4) young fruits approximately 10 days post-fertilization (length: circa 1 cm); (5) mature fruits approximately 60 days (Cakile) and 25 days (Erucaria) post-fertilization (1.8 cm long per Cakile, 1.5 cm long per Erucaria); and (6) leaves. Fruits were not dissected between distal and proximal because both segments contain elements of the joint. RNA was extracted using Concert Plant RNA Reagent kit (Invitrogen, Carlsbad, CA, USA) then DNAse treated to remove DNA (TURBO DNA-free kit, Ambion). cDNA was then synthesized from 2 μg RNA using SuperScriptIII reverse transcriptase (Invitrogen, Carlsbad, CA, USA). RT-PCR reactions were carried out using locus-specific primers (Additional file 1: TableS1), 1.0 U of rTaq or exTaq DNA polymerase (Takara Co., Tokyo, Japan) in 25 μL of PCR buffer (20 mM Tris‐HCl pH 8, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.5% Tween®20, 0.5% Nonidet® P-40, 50% Glycerol) containing 30 pmol of 5′ and 3′ primers, 200 μM of each dNTP, and 0.5-1 μL of cDNA. Amplification program was as follows: 94° for 2 min, followed by 15 to 27 cycles of 94° for 20 s, 50° to 55° for 30 s, and 72° for 1 min. We established the linear range of amplification by testing different cycle durations. No bands were discerned for genes of interest at 15 cycles, weak bands were observed at 20 in Cakile (but not Erucaria), moderate bands at 22, and strong bands at 27. The 27 band results are shown in the text but the 20 and 22 are also shown in the supplement. Reactions were run on a 1.2% agarose gel and digitally photographed using Alpha Innotech’s Red™ Imaging System (San Leandro, CA, USA). The identity of amplified fragments was confirmed by amplicon size and sequencing.
Identification of the valve margin pathway genes
To establish the possible roles of valve margin pathway genes in the development and evolution of heteroarthrocarpy, we identified homologs of ALC, FUL, IND, RPL, and SHP1/SHP2 from Cakile (Cl-) and Erucaria (Ee-). Screening of putative orthologs revealed one copy of every gene except FUL, which had two copies in both focal species (GenBank: JX292959-JX292970). SHP1 was not identified from Erucaria and IND was not found in Cakile cDNA. Thus, 12 loci were identified in total: ClALC, ClFUL1, ClFUL2, ClRPL, ClSHP1, ClSHP2, EeALC, EeFUL1, EeFUL2, EeIND, EeRPL, and EeSHP2. Gene identity was confirmed by seParate phylogenetic analyses of AGAMOUS, APETALA1/FUL, bHLH, and BEL-like sequences from a range of angiosperms (Additional file3: Figure S1, Additional file4: Figure S2, Additional file5: Figure S3, and Additional file6: Figure S4, Additional file2: Table S2). In all instances, the putative homologs from Cakile and Erucaria were closely related to genes from other Brassicaceae, specifically Arabidopsis and Brassica (for example, ClALC plus EeALC are sister to ALC; Additional file3: Figure S1, Additional file4: Figure S2, Additional file5: Figure S3, and Additional file6: Figure S4).
Although the tribe Brassiceae has experienced an ancient triploidization[41, 42], only two duplications were observed in Cakile and Erucaria among the 12 SHP-, FUL-, ALC-, IND, and RPL- like genes identified. The Brassicaceae-specific duplication of SHP1/SHP2[43, 44] was recovered in Cakile, but not Erucaria. Additional studies of Erucaria are needed to determine if this represents a real loss of SHP1. Importantly, two copies of FUL were uncovered in both focal taxa. These findings are somewhat consistent with that of Brassica, the only other taxon within the tribe for which sequence information is available. In Brassica, only one copy of IND is present, whereas multiple copies of FUL, RPL, and SHP are observed (Additional file 3: FigureS1, Additional file4: Figure S2, Additional file5: Figure S3, and Additional file6: Figure S4;[23, 45]).
Valve margin pathway expression data in Erucaria erucarioides
The expression domain of EeFUL1 was restricted to the valves of developing flowers and fruits. Early in development, EeFUL1 expression was observed in the inner tissue of developing valves (Figure2l). After layers of the ovary wall had differentiated, EeFUL1 had a specific expression domain in the inner layer of the mesocarp and the endb layer (Figure2m), but only of the proximal segment (Figure2n). Occasionally, expression of EeFUL1 was also observed in the ovules and nectaries of young and old buds (Additional file7: Figures S5a to b). EeFUL2 had a markedly different expression profile than EeFUL1 with expression observed in the ovules and nectaries of young and old buds, but not in the valves (Additional file7: Figure S5c).
Valve margin pathway expression data in Cakile lanceolata
We also conducted RT-PCR on all identified loci to establish expression patterns that we were not able to observe with in situ hybridization (Figure3b). Similar to results with Erucaria, we assessed expression based on 27 cycles (see Additional file7: Figure S5d for lower cycle numbers). Consistent with in situ hybridization, ClSHP1 and ClSHP2 expression was observed in young buds through fruit maturation. ClALC expression was observed at earlier developmental stages (for example, young buds; Figure3b) with RT-PCR than was observed with in situ hybridization. Contrary to what was observed with in situ hybridization, RT-PCR results indicated that ClFUL1, ClFUL2, and ClRPL were expressed across most developmental stages and tissue types with few exceptions. ClRPL, ClSHP2, and ClALC were not detected in leaves whereas ClFUL1 was not detected in young buds.
Dehiscence in Erucaria
In the critical valve dehiscence zone of the proximal segment of Erucaria, the expression domains of the Erucaria SHP1/2, ALC, and IND homologs are largely conserved relative to what has been observed in Arabidopsis and Brassica[14, 17–19, 46, 47]. Outside the valve margin, some differences from Arabidopsis were found in expression of EeIND and EeALC, but the functional significance of these minor differences remains to be determined. Perhaps most intriguing is the expression of the Erucaria FUL homologs. In Arabidopsis, FUL is expressed throughout developing valve tissue and has a loss of function phenotype that exhibits defects in the differentiation and expansion of the valve, which is caused in part by an expansion of the SHP1/2, IND, and ALC expression domains. The two copies of FUL from Erucaria have very different expression patterns from each other. EeFUL1 is expressed in the mesocarp and endb layer of developing valve walls in the proximal segment (Figure2l to n), implying possible conservation in the role of EeFUL1 in limiting the expression of EeSHP2, EeIND, and EeALC to the valve margin. In contrast, EeFUL2 is expressed in developing ovules and nectaries, but not in the ovary wall. Given that Arabidopsis FUL is also expressed in nectaries but not ovules, the novel EeFUL2 expression pattern likely indicates a combination of neo- and subfunctionalization or, alternatively, non-specific staining with our EeFUL2 probes. Overall, however, the EeFUL1 expression pattern is the most relevant in that it provides further evidence for the complex identity of the ovary wall in Erucaria and how heteroarthrocarpy relates to the typical silique of Arabidopsis. Hall et al. (2006) previously hypothesized that the valves of heteroarthrocarpic fruits are disassociated from the repla. This essentially means that although the replum and septum develop throughout the entire length of the fruit, only a basal region of the ovary wall differentiates as valve. If we treat EeFUL1 expression as a kind of marker for valve identity, consistent with FUL function in Arabidopsis, these expression data would appear to confirm the hypothesis in showing that FUL homolog expression has contracted in Erucaria to encompass only the dehiscent portion of the ovary wall. This new domain seems to distinguish the functional valve region, which will dehisce and fall off, from the indehiscent portion of the ovary wall, which will form the persistent pericarp of the distal segment.
Unfortunately, no expression of EeRPL was observed in developing flowers and carpels with in situ hybridization, although RT-PCR indicates it is expressed in developing flowers and fruits (Figure3a). This result was somewhat surprising as RPL, also known as PENNYWISE (PNY), BELLRINGER (BLR), and VAAMANA (VAN), is broadly expressed in developing Arabidopsis meristems due to an additional role in determining internode elongation[50, 51]. More important to fruit development, in Arabidopsis, RPL negatively regulates FIL, JAG, and YAB3, three genes that redundantly function to activate FUL and SHP. Because we have not been able to adequately capture RPL expression in this species with in situ hybridization, we cannot determine if there may be conservation between Arabidopsis and Erucaria on where the gene is expressed in developing carpels. Examination of JAG, FIL, and YAB3 would also be informative. Fruits of fil yab3 mutants lack a valve margin in the distal portion and have ectopic expression of valve margin in the proximal portion, which is somewhat reminiscent of heteroarthrocarpy.
Indehiscence is correlated with loss of gene expression in the ovary wall
Indehiscence of the proximal segment of Cakile is associated with complete loss of gene expression of the valve margin pathway in the ovary wall and presumptive valve margin (Figure5). Indehiscence of distal segments is also correlated with the absence of SHP2, ALC, and FUL expression in the ovary wall of Erucaria and the absence of SHP1/SHP2 in Cakile (Figures2,4). This pattern is likely the result of this fruit segment having a completely novel developmental program when compared to Arabidopsis. In addition to redundant roles in promoting valve margin identity, SHP1/SHP2 have partial redundancy in ovule development with AGAMOUS and SEEDSTICK in Arabidopsis. As such, expression was maintained in the ovules of indehiscent segments of Cakile and Erucaria even when not observed in the carpel wall. A similar compelling pattern is observed with EeALC, EeIND, and EeFUL1. Expression of all three genes is observed in the presumptive valve margin or valve in dehiscent proximal segment, but not the indehiscent distal, thus highlighting the correlation of indehiscence with elimination of gene expression in the ovary wall. Our inability to visualize ClFUL and ClRPL expression in developing carpels, as suggested by RT-PCR data, means that we cannot assess their potential roles in the loss of SHP expression. Regardless, the current study suggests that indehiscence may be the result of the elimination of SHP expression in the developing fruit wall.
Recruitment of valve margin pathway in joint abscission is unclear
No expression of any valve margin pathway gene was observed in the joint of either focal species, which is especially important when considering the abscising joint of Cakile. While it is possible that this pathway is simply not functioning in the joint, we must also consider the likelihood that difficulties in detecting expression of these loci have impaired our ability to assess their patterns. For example, it is possible that we have failed to capture homologs that may be expressed in the joint region. ALC, IND, and RPL have largely been characterized via reporter lines in Arabidopsis[14, 18, 20], suggesting that expression of these genes is challenging to determine even in model species. Thus we cannot eliminate with confidence the possibility that these genes are being expressed in the joint, which represents a modification in the distal portion of the valve margin. Additionally, joint abscission occurs via the juxtaposition of non-lignified and lignified cells, which is an identical pattern to valve dehiscence. This anatomy may reflect differential expression of an otherwise conserved valve margin pathway that could involve ALC and IND but not SHP1/2 and RPL. The maintenance and expression of ClFUL1 and ClFUL2 in these indehiscent fruits is perplexing (Figure3b), but suggest that these genes may be involved in joint abscission, even if they are not required for dehiscence. Alternatively, FUL may function to repress dehiscence zones in Cakile, similar to the role of FUL in Arabidopsis. However, we are currently unable to distinguish between these hypotheses due to technological issues with in situ hybridization.
Heteroarthrocarpy and its variants have evolved multiple times across the tribe, implying lability in the underlying genetic pathway for this unusual fruit type. Expression data from two variants of heteroarthrocarpy that represent a single evolutionary origin provide insight into how the valve margin pathway may have been modified. First, conservation in the expression patterns of genes promoting valve margin identity in the dehiscent segment of Erucaria was observed, suggesting these genes maintain the same functions as in Arabidopsis. In contrast, expression patterns of genes involved in positioning that pathway were different between Erucaria and Arabidopsis. These observations support anatomical data proposing that heteroarthrocarpy is the result of repositioning the valve margin, however they are not completely informative in precisely how the pathway has been repositioned. Additionally, indehiscence in all segments was characterized by the elimination of valve margin pathway and positioning genes. The absence of expression likely has a different developmental basis between distal and proximal segments as the ovary wall of distal segments does not differentiate into valves. Finally, the genetic basis of the joint remains elusive. Regardless, it is clear that modifications in expression patterns of the valve margin pathway are correlated with indehiscence and segmentation in Cakile and Erucaria.
- end b endocarp b:
b endocarp b
reverse transcription-polymerase chain reaction
We thank T Tisdale, A Wheeler, and members of the Hall lab for assistance with plant care; P Mankowski provided excellent graphical support; S McCrea helped with RT-PCR experiments. This work was supported by a Mercer Post-Doctoral Fellowship, Harvard University, to JCH, an Alberta Ingenuity New Faculty award to JCH, a NSERC Discovery grant to JCH, and the William F Milton Fund, Harvard University, to KD.
- Hall JC, Donohue K: Genetics of Plant Dispersal. Dispersal: causes and consequences. Edited by: Clobert J, Baguette M, Benton T, Bullock J. New York, NY: Oxford University Press, in press
- Bolmgren K, Eriksson O: Fleshy fruits - origins, niche shifts, and diversification. Oikos. 2005, 109: 255-272.View ArticleGoogle Scholar
- Lorts CM, Briggeman T, Sang T: Evolution of fruit types and seed dispersal: A phylogenetic and ecological snapshot. J Syst Evol. 2008, 46: 396-404.Google Scholar
- Knapp S: Tobacco to tomatoes: a phylogenetic perspective on fruit diversity in the Solanaceae. J Exp Bot. 2002, 53: 2001-2022.View ArticlePubMedGoogle Scholar
- Clausing G, Meyer K, Renner SS: Correlations among fruit traits and evolution of different fruits within Melastomataceae. Bot J Linn Soc. 2000, 133: 303-326.View ArticleGoogle Scholar
- Appel O, Al-Shehbaz IA: Cruciferae. The Families and Genera of Vascular Plants. Volume V. Flowering plants, Dicotyledons: Malvales, CapParales, and Non-betalain Caryophyllales. Edited by: Kubitzki K, Bayer C. 2003, Berlin: Springer, 75-174.Google Scholar
- Koch M, Al-Shehbaz IA, Mummenhoff K: Molecular systematics, evolution, and population biology in the mustard family (Brassicaceae). Ann Mo Bot Gard. 2003, 90: 151-171.View ArticleGoogle Scholar
- Mummenhoff K, Polster A, Muhlhausen A, Theissen G: Lepidium as a model system for studying the evolution of fruit development in Brassicaceae. J Exp Bot. 2009, 60: 1503-1513.View ArticlePubMedGoogle Scholar
- Doyle J, Luckow M: The rest of the iceberg. Legume diversity and evolution in a phylogenetic context. Plant Physiol. 2003, 131: 900-910.PubMed CentralView ArticlePubMedGoogle Scholar
- Lewis MW, Leslie ME, Liljegren SJ: Plant seParation: 50 ways to leave your mother. Curr Opin Plant Biol. 2006, 9: 59-65.View ArticlePubMedGoogle Scholar
- Dinneny JR, Weigel D, Yanofsky MF: A genetic framework for fruit patterning in Arabidopsis thaliana. Development. 2005, 132: 4687-4696.View ArticlePubMedGoogle Scholar
- Dinneny JR, Yanofsky MF: Drawing line and borders: how the dehiscent fruit of Arabidopsis is patterned. Bioessays. 2004, 27: 42-49.View ArticleGoogle Scholar
- Spence J, Vercher Y, Gates P, Harris N: ‘Pod shatter’ in Arabidopsis thaliana Brassica napusandB. juncea. J Microsc-Oxf. 1996, 181: 195-203.View ArticleGoogle Scholar
- Liljegren SJ, Roeder AHK, Kempin SA, Gremski K, Østergaard L, Guimil S, Reyes DK, Yanofsky MF: Control of fruit patterning in Arabidopsis by INDEHISCENT. Cell. 2004, 116: 843-853.View ArticlePubMedGoogle Scholar
- Flanagan CA, Hu Y, Ma H: Specific expression of the AGL1 MADS-box gene suggests regulatory functions in Arabidopsis gynoecium and ovule development. Plant J. 1996, 10: 343-353.View ArticlePubMedGoogle Scholar
- Savidge B, Rounsley SD, Yanofsky MF: Temporal relationship between the transcription of two Arabidopsis MADS box genes and the floral organ identity genes. Plant Cell. 1995, 7: 721-733.PubMed CentralView ArticlePubMedGoogle Scholar
- Liljegren SJ, Ditta GS, Eshed Y, Savidge B, Bowman JL, Yanofsky MF: SHATTERPROOF MADS-box genes control seed dispersal in Arabidopsis. Nature. 2000, 404: 766-770.View ArticlePubMedGoogle Scholar
- Rajani S, Sundaresan V: The Arabidopsis myc/bHLH gene ALCATRAZ enables cell seParation in fruit dehiscence. Curr Biol. 2001, 11: 1914-1922.View ArticlePubMedGoogle Scholar
- Ferrandiz C, Liljegren SJ, Yanofsky MF: Negative regulation of the SHATTERPROOF genes by FRUITFULL during Arabidopsis fruit development. Science. 2000, 289: 436-438.View ArticlePubMedGoogle Scholar
- Roeder AHK, Ferrandiz C, Yanofsky MF: The role of the REPLUMLESS homeodomain protein in patterning of Arabidopsis fruit. Curr Biol. 2003, 13: 1630-1635.View ArticlePubMedGoogle Scholar
- Alonso-Cantabrana H, Ripoll JJ, Ochando I, Vera A, Ferrandiz C, Martinez-Laborda A: Common regulatory networks in leaf and fruit patterning revealed by mutations in the Arabidopsis ASYMMETRIC LEAVES1 gene. Development. 2007, 134: 2663-2671.View ArticlePubMedGoogle Scholar
- Ripoll JJ, Roeder AHK, Ditta GS, Yanofsky MF: A novel role for the floral homeotic gene APETALA2 during Arabidopsis fruit development. Development. 2011, 138: 5167-5176.View ArticlePubMedGoogle Scholar
- Arnaud N, Lawrenson T, Ostergaard L, Sablowski R: The same regulatory point mutation changed seed-dispersal structures in evolution and domestication. Curr Biol. 2011, 21: 1215-1219.View ArticlePubMedGoogle Scholar
- Girin T, Stephenson P, Goldsack CMP, Kempin SA, Perez A, Pires N, Sparrow PA, Wood TA, Yanofsky MF, Østergaard L: Brassicaceae INDEHISCENT genes specify valve margin cell fate and repress replum formation. Plant J. 2010, 63: 329-338.View ArticlePubMedGoogle Scholar
- Ostergaard L, Kempin SA, Bies D, Klee HJ, Yanofsky MF: Pod shatter-resistant Brassica fruit produced by ectopic expression of the FRUITFULL gene. Plant Biotechnol J. 2006, 4: 45-51.View ArticlePubMedGoogle Scholar
- Bailey CD, Koch MA, Mayer M, Mummenhoff K, O’Kane SL, Warwick SI, Windham MD, Al-Shehbaz IA: Toward a global phylogeny of the Brassicaceae. Mol Biol Evol. 2006, 23: 2142-2160.View ArticlePubMedGoogle Scholar
- Warwick SI, Sauder CA: Phylogeny of tribe Brassiceae (Brassicaceae) based on chloroplast restriction site polymorphisms and nuclear ribosomal internal transcribed spacer and chloroplast trnL intron sequences. Can Journal Bot-Rev CanDe Botanique. 2005, 83: 467-483.Google Scholar
- Hall JC, Tisdale TE, Donohue K, Wheeler A, Al-Yahya MA, Kramer EM: Convergent evolution of a complex fruit structure in the tribe Brassiceae (Brassicaceae). Am J Bot. 2011, 98: 1989-2003.View ArticlePubMedGoogle Scholar
- Hall JC, Tisdale TE, Donohue K, Kramer EM: Developmental basis of an anatomical novelty: heteroarthrocarpy in Cakile lanceolata and Erucaria erucarioides (Brassicaceae). Int J Plant Sci. 2006, 167: 771-789.View ArticleGoogle Scholar
- Appel O: The so-called ‘beak’, a character in the systematics of Brassicaceae?. Bot Jahrb Syst Pflanzengesch Pflanzengeogr. 1999, 121: 85-98.Google Scholar
- Zohary M: Carpological stuides in Cruciferae. Palest J Bot, Jerusalem Series. 1948, 4: 158-165.Google Scholar
- Imbert E: Ecological consequences and ontogeny of seed heteromorphism. Perspect Plant Ecol Evol Syst. 2002, 5: 13-36.View ArticleGoogle Scholar
- Rodman JE: Systematics and evolution of the genus Cakile (Cruciferae). Contrib from the Gray Herbarium. 1974, 205: 3-146.Google Scholar
- Donohue K: Maternal determinants of seed dispersal in Cakile edentula: Fruit, plant, and site traits. Ecology. 1998, 79: 2771-2788.View ArticleGoogle Scholar
- Taghizadeh MS, Crawford S, Nicolas ME, Cousens RD: Water deficit changes the anatomy of the fruit abscission zone in Raphanus raphanistrum (Brassicaceae). Aust J Bot. 2009, 57: 708-714.View ArticleGoogle Scholar
- Kramer EM, Dorit RL, Irish VF: Molecular evolution of genes controlling petal and stamen development: Duplication and divergence within the APETALA3 and PISTILLATA MADS-box gene lineages. Genetics. 1998, 149: 765-783.PubMed CentralPubMedGoogle Scholar
- Hall AE: BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl Acids Symp Ser. 1999, 41: 95-98.Google Scholar
- Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S: MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol. 2011, 28: 2731-2739.PubMed CentralView ArticlePubMedGoogle Scholar
- Kramer EM: Methods for studying the evolution of plant reproductive structures: ComParative gene expression techniques. Molecular Evolution: Producing the Biochemical Data, Part B. Methods in Enzymology, volume 395. Edited by: Zimmer EA, Waltham RE. 2005, MA: Academic, 617-636.View ArticleGoogle Scholar
- Jackson D: In situhybridisation in plants. Molecular plant pathology, a practical approach. Edited by: Bowles DJ, Gurr SJ, McPherson P. 1991, Oxford: Oxford University PressGoogle Scholar
- Lysak MA, Koch MA, Pecinka A, Schubert I: Chromosome triplication found across the tribe Brassiceae. Genome Res. 2005, 15: 516-525.PubMed CentralView ArticlePubMedGoogle Scholar
- Parkin IAP, Gulden SM, Sharpe AG, Lukens L, Trick M, Osborn TC, Lydiate DJ: Segmental structure of the Brassica napus genome based on comParative analysis with Arabidopsis thaliana. Genetics. 2005, 171: 765-781.PubMed CentralView ArticlePubMedGoogle Scholar
- Kramer EM, Jaramillo MA, Di Stilio VS: Patterns of gene duplication and functional evolution during the diversification of the AGAMOUS subfamily of MADS box genes in angiosperms. Genetics. 2004, 166: 1011-1023.PubMed CentralView ArticlePubMedGoogle Scholar
- Zahn LM, Leebens-Mack JH, Arrington JM, Hu Y, Landherr LL, dePamphilis CW, Becker A, Theissen G, Ma H: Conservation and divergence in the AGAMOUS subfamily of MADS-box genes: evidence of independent sub- and neofunctionalization events. Evol Dev. 2006, 8: 30-45.View ArticlePubMedGoogle Scholar
- Tan XL, Xia ZW, Zhang LL, Zhang ZY, Guo ZJ, Qi CK: Cloning and sequence analysis of oilseed rape (Brassica napus) SHP2 gene. Bot Stud. 2009, 50: 403-412.Google Scholar
- Mandel MA, Yanofsky MF: The Arabidopsis AGL8 MADS-Box gene is expressed in inflorescence meristems and is negatively regulated by APETALA1. Plant Cell. 1995, 7: 1763-1771.PubMed CentralView ArticlePubMedGoogle Scholar
- Sorefan K, Girin T, Liljegren SJ, Ljung K, Robles P, Galvan-Ampudia CS, Offringa R, Friml J, Yanofsky MF, Østergaard L: A regulated auxin minimum is required for seed dispersal in Arabidopsis. Nature. 2009, 459: 583-586.View ArticlePubMedGoogle Scholar
- Gu Q, Ferrandiz C, Yanofsky MF, Martienssen R: The FRUITFULL MADS-box gene mediates cell differentiation during Arabidopsis fruit development. Development. 1998, 125: 1509-1517.PubMedGoogle Scholar
- Force A, Lynch M, Pickett FB, Amores A, Yan YL, Postlethwait J: Preservation of duplicate genes by complementary, degenerative mutations. Genetics. 1999, 151: 1531-1545.PubMed CentralPubMedGoogle Scholar
- Smith HMS, Hake S: The interaction of two homeobox genes, BREVIPEDICELLUS and PENNYWISE, regulates internode patterning in the Arabidopsis inflorescence. Plant Cell. 2003, 15: 1717-1727.PubMed CentralView ArticlePubMedGoogle Scholar
- Byrne ME, Groover AT, Fontana JR, Martienssen RA: Phyllotactic pattern and stem cell fate are determined by the Arabidopsis homeobox gene BELLRINGER. Development. 2003, 130: 3941-3950.View ArticlePubMedGoogle Scholar
- Pinyopich A, Ditta GS, Savidge B, Liljegren SJ, Baumann E, Wisman E, Yanofsky MF: Assessing the redundancy of MADS-box genes during carpel and ovule development. Nature. 2003, 424: 85-88.View ArticlePubMedGoogle Scholar
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