The diversity, development and evolution of polyclad flatworm larvae
© Rawlinson; licensee BioMed Central Ltd. 2014
Received: 20 November 2013
Accepted: 23 January 2014
Published: 6 March 2014
Polyclad flatworms offer an excellent system with which to explore the evolution of larval structures and the ecological and developmental mechanisms driving flatworm and marine invertebrate life history evolution. Although the most common mode of development in polyclads might be direct development (where the embryo develops directly into a form resembling the young adult), there are many species that develop indirectly, through a planktonic phase with transient larval features, before settling to the sea floor. In this review, I introduce polyclad life history strategies, larval diversity and larval anatomical features (presenting previously unpublished micrographs of a diversity of polyclad larvae). I summarize what is known about polyclad larval development during the planktonic phase and the transition to the benthic juvenile. Finally, I discuss evolutionary and developmental scenarios on the origin of polyclad larval characters.
The most prominent characters that are found exclusively in the larval stages are lobes that protrude from the body and a ciliary band, or ciliary tufts, at the peripheral margins of the lobes. Larvae with 4–8 and 10 lobes have been described, with most indirect developing species hatching with 8 lobes. A ventral sucker develops in late stage larvae, and I put forward the hypothesis that this is an organ for larval settlement for species belonging to the Cotylea. Historically, the biphasic life cycle of polyclads was thought to be a shared primitive feature of marine invertebrates, with similarities in larval features among phyla resulting from evolutionary conservation. However, our current understanding of animal phylogeny suggests that indirect development in polyclads has evolved independently of similar life cycles found in parasitic flatworms and some other spiralian taxa, and that morphological similarities between the larvae of polyclads and other spiralians are likely a result of convergent evolution.
KeywordsPolyclads Platyhelminthes Larvae Meroplankton Lobes Ciliary band Sucker
Jägersten  considered the biphasic life cycle of polyclads to be the ancestral condition for Platyhelminthes. He also considered polyclad larvae to be primary larvae (a free-swimming larval stage that was descended directly from that of the last common ancestor of the metazoans) and neodermatan larvae as secondary larvae (a larval phase evolved secondarily from a direct-developing ancestor). In other words, polyclad and neodermatan larvae were thought to have evolved independently, with polyclad larval characters being primitive features shared between phyla with primary larvae (for example, cyphonautes (Bryozoa), trochophora (Annelida, Mollusca), actinotrocha (Phoronida), tornaria (Hemichordata), auricularia (Echinodermata)), and neodermatan larval characters having evolved de novo. Over the last four decades, our knowledge of metazoan phylogeny and larval morphology has increased significantly, and we can now revisit these hypotheses in light of new data. This review examines polyclad larval diversity, development and evolution by summarizing classical literature, synthesizing recent work and presenting new data. This review also establishes a foundation for future studies of metazoan larval character evolution.
Polyclad life history strategies and larval diversity
Polyclad species with indirect development from published literature
Number of larval lobes
Length at hatching, μm
95 to 105
85 to 95
4 to 5
90 to 100
170 to 185
Müller’s: intracapsular & free-swimming (known as Kato’s larva)
310 to 330
250 to 300
Culturing polyclad larvae to metamorphosis is difficult and rarely achieved, so much of our understanding of this life history stage has come from plankton tows. For example, development over the larval stage and metamorphosis has been illustrated based on specimens collected from the plankton [14, 37], and a higher diversity of larval forms have been captured in plankton nets [11, 38, 39] than have been described from laboratory cultures. The water column is a relatively under-sampled environment for polyclads, which generally undergo embryogenesis and spend their adult life on the sea floor. Consequently, sampling coastal waters (which are rich in meroplankton - the larval stages of organisms that will become nektonic or benthic as adults) may lead to the discovery of new larval types, an increased understanding of the developmental processes that take place over the planktonic stage, and new hypotheses on the evolution of biphasic life cycles.
Lang  proposed that the Götte’s larva was an earlier developmental stage of the Müller’s larva, with two more pairs of lateral lobes developing during the larval phase. However, it is now widely accepted that the Götte’s larva is a distinct larval form from the Müller’s larva, as many species that hatch with four lobes never develop any further lobes [6, 25] (with the exception of Stylochus tauricus which was described to develop a fifth lobe 7 days post hatching , although it is not clear from their illustrations where this lobe is positioned). There is evidence that in some Müller’s larvae, further lobes may develop over the larval period. For example, at hatching, the cotylean Pseudoceros canadensis has six lobes [28, 29] but a pair of dorso-lateral lobes develops later . Furthermore, there are no observations of species hatching with ten lobes, which led Dawydoff  to speculate that plankton caught with ten lobes hatch with eight, and gain additional lobes during the larval period.
Polyclad larval characters: morphology, development and function
The larval stages of benthic marine invertebrates have classically been regarded as morphologically, ecologically and physiologically distinct from the juvenile and adult stages. However, it is now clear that in some spiralian taxa, many features of the larval body plan persist into the adult body plan, with the larval period defined by the presence of a collection of transient “larval characters” [46–49]. With respect to polyclad flatworms, we are in the early stages of determining which characters are specific to the larval stage. The most obvious larva-specific features are the lobes and ciliary bands or tufts. Other characters, such as the frontal (or apical) organ, are also reported to be lost during metamorphosis [6, 37]. More detailed studies on the metamorphosis of indirect-developing species, and comparative analyses of indirect and direct-developing taxa, are needed to distinguish between characters that are unique to the larval stage, and those that persist through the larval stage and into the juvenile/adult.
Larval lobes are temporary protrusions  made up of epidermal cells (including prototrochal cells, ciliated epidermal cells, monociliated sensory cells and neurons ), body wall muscle [50, 51] and connective tissue/extracellular matrix  (Figure 2B). Unlike the rest of the animal the lobes lack epidermal rhabdites and yolk  (Figure 2B). Outgrowth of the lobes occurs relatively late in embryogenesis (during the last 24 hours prior to hatching in Maritigrella crozieri, ). The long lobes found in larvae collected from the plankton would suggest that lobe growth continues in the water column (Figure 3B, D, F). The cellular mechanisms that drive the outgrowth of the lobes are unknown, as is the fate of lobe tissue during resorption/settlement. The body wall muscle (and other tissue) of the lobes may be incorporated into the body of the juvenile and adult.
At the peripheral margins of the lobes, cells with longer cilia form continuous, pre-oral ciliary bands (often called prototroch) (such as Maritigrella crozieri,[52, 53]), discontinuous bands (Pseudoceros canadensis), or numerous distinct bands or tufts (Imogine mcgrathi; Amakusaplana acroporae) (Figure 2C-F). Further analysis is needed to investigate the variation in the configuration of the ciliary bands among species, and to assess its functional significance. In M. crozieri, the ciliary band is formed by hundreds of prototrochal cells, positioned in rows  and innervated by a dense sub-cilial plexus of serotonin and FMRFamide immunoreactive cells . The ciliary band forms shortly after gastrulation as a ring around the developing stomodaeum at the posterior end of the embryo (in M. crozieri; ). As the stomodaeum migrates ventrally, the ciliary band becomes convoluted and migrates to the position of the future larval lobes. In P. canadensis cells with longer cilia are also described from the sub-oral plate. They are continuous with the ciliary band that follows the lobes and Lacalli  calls these rejectory cells and assumes a function in food rejection (Figure 2E). It is possible that these cells are found in M. crozieri (see Figure seven D in ) and A.acroporae (Figure six D in ) larvae as well (Figure 2A, F).
Polyclad larvae swim continuously , and metachronal waves propagate around the ciliary band providing the main propulsive force . The ciliated bands are also used for feeding; with cilia on the lobes beating in the direction of the larval body, cilia on the ventral surface beating towards the mouth and specialized club-shaped cilia hypothesized to function as a valve in a ring at the stomodaeum-gut junction .
Although there are no direct observations of Müller’s and Götte’s larvae feeding, phytoplankton was observed in the gut lumen of eight-lobed Müller’s larvae [30, 56], with greater survival times in fed compared to unfed larvae . Götte’s larvae, on the other hand, are considered lecithotrophic [25, 37], or facultative planktotrophs (it has been reported that Imogine mcgrathi feeds passively on microplankton ). Comparisons of body shape and placement of cilia on marine larval bodies reveal that there is a tendency for feeding larvae to have complex body shapes, and for nonfeeding larvae to have simple body shapes . These correlations of body shape with trophic status seem to apply for polyclad larvae: Müller’s larvae, with complex and large body shapes, are probably active planktotrophs, whereas the small, simple Götte’s larvae are facultative feeders or lecithotrophs.
At the anterior pole of polyclad larvae is a tuft of cilia and associated gland cells. These have been named a frontal organ [6, 37], apical organ  or apical plate [45, 52], and a glandulo-sensory function has been suggested . We do not have a very complete picture of this organ’s anatomy and function, as different studies have used different methods and different species, so comparison across polyclad taxa is difficult. I shall call this organ a frontal organ to avoid suggesting that it is homologous to the apical organ found in other spiralian larval stages, as we do not have sufficient data yet to make these comparisons. Histologically, the frontal organ looks like a columnar mass of connective tissue between the brain and the apical cilia  (Figure 2Bii). Ultrastructural studies [28, 37, 58] show that the frontal organ consists of a cluster of monociliated or multicilated sensory cells (in Müller’s and Götte’s larvae respectively ), surrounded by a circle of gland-cell necks supported peripherally by microtubules. The gland-cell necks pass through the basement membrane extending to secretory-cell bodies around the brain [28, 37]. The bases of the monociliated sensory cells in Pseudoceros canadensis are located on top of the brain, but no direct connection to the brain has been observed. Lacalli  therefore determined that these cells were not nerve cells. Immunohistochemical and immunofluorescent studies [45, 52] show immunoreactivity in the form of an apical plate. Antibodies against the neurotransmittors serotonin (5HT) and FMRFamide are expressed in a ring around the apical cilia in M. crozieri and these expression patterns may correspond to the monociliated sensory cells of P. canadensis. There appears to be little 5HT or FMRFamide immunoreactivity in the rest of the organ . These preliminary findings may suggest that the frontal organ may be more glandular than sensory, but that it is surrounded by neural activity in the brain and apical plate.
Kato  suggested that the frontal organ is used to break the eggshell at hatching and noted that it degenerates 1 to 2 days post hatching in Planocera reticulata (which has already undergone metamorphosis inside the egg capsule). If the frontal organ does have a role in breaking the eggshell, then perhaps it is also found in direct-developing hatchlings. Apical cilia have been identified in direct-developing hatchlings  and comparative studies between direct- and indirect-developing species would determine whether the frontal organ is a character unique to indirect developers. Ruppert  suggests that the frontal organ may play a role in larval settlement, similar to the organ of larval attachment in larvae of the ectoproct Loxosomella. There are many fundamental questions on the morphology, function and evolution of the frontal organ in polyclads that need to be answered. Ideally, in one species, we would use scanning and transmission electron microscopy, immunostaining and gene expression analysis to understand the relationship between the apical cilia, the gland cells and their innervation. We would also carry out experiments to test whether the frontal organ functions as a specialized sensory-neurosecretory structure that detects environmental cues and transduces them into behavioral responses (for example, settlement).
At hatching, ciliary-band beating and activity of longitudinal, circular and diagonal muscles to steer the larvae are coordinated by sensory and motor nerves centralized through the brain . Light-sensing organs in the form of pigmented rhabdomeric photoreceptors and putative non-pigmented extra-ocular photoreceptors [28, 45, 52] (Figure 2) detect light information, allowing the larva to assess its depth, detect shadows of predators and swim accordingly. These light-sensing organs are, however, not specific to the larval phase and become more numerous and modified in the juvenile and adult stages ( and Rawlinson (unpublished data)). A study on polyclad larval photobehavior demonstrated that M. crozieri larvae are relatively insensitive to light (compared to other invertebrate larvae) but that their visual sensitivity increases with age . They display an ontogenetic pattern in which young larvae are positively phototactic at high light-intensities and negative at low (a pattern typical of a predator avoidance shadow-response, which describes an animal’s defensive response to a sudden decrease in illumination), while older larvae are only positively phototactic (a behavior suggested to be beneficial for use of near surface tidal currents for recruitment to shallow adult habitats) .
Development in the water column and transition to the benthic juvenile stage
Planktonic larvae commonly pass through an extended period of development before they become competent to settle and metamorphose . Much of the growth of feeding larvae occurs during this precompetent period [61–63]. Is feeding necessary for polyclad larvae to grow and achieve metamorphic competence? In the absence of food, the four-lobed larvae of Notoplana australis and Stylochus uniporus settled and metamorphosed after a short planktonic phase (two weeks and 4 to 5 days respectively [6, 25]). For Müller’s larvae, yolk is still present at hatching (Figure 2), so perhaps there is a period of facultative planktotrophy (and possibly obligatory planktotrophy, depending on the duration of the larval stage and extent of yolk reserves in different species) [14, 37]. Müller’s larva of some species may spend prolonged periods in the plankton . The larval period for M. crozieri is estimated to be at least 3.5 weeks , and at least 40 days for P. canadensis and Stylostomum sanjuania. For species with intermediate development, intra-capsular larvae are lecithotrophic [5, 6] and adelphophagy has been observed .
The transition from a slightly laterally flattened planktonic larva to the dorso-ventally flattened benthic adult involves changes in the mode of feeding and locomotion, from ciliary to muscular in both cases. This transition in polyclads is gradual [14, 37], and involves the development of the gut diverticula, a plicate pharynx, and diagonal body wall, dorso-ventral, parenchymal and sucker muscles [37, 52]. In some marine invertebrate larvae, metamorphic competence is reached when all requisite juvenile characters are present in the larva, and thus metamorphosis is essentially the loss of larva-specific structures and physiological processes . We know so little about development in the larval and early juvenile stages that we have no understanding of larval competence in polyclads. However, evidence of competence might be the presence of a developed sucker on the ventral surface in some species (see below) and changes in phototactic behavior. Some lecithotrophic species may be competent at hatching, as is typical of most lecithotrophic Porifera, Cnidaria, Bryozoa with coronate larvae, colonial Ascidiacea and many polychaetes .
A caudal adhesive organ (sucker) develops prior to metamorphosis in cotylean polyclads (Thysanozoon and Yungia species; [14, 37]), and this was found commonly in eight- and ten-lobed larvae collected from the plankton (Figures 3Eii and 4). No hatchling or early larval stages have been reported to have a sucker, so this organ probably develops later in the planktonic phase. While its function in adult cotylean species is suggested to be a holdfast against the action of waves and currents , its role in larval stages has not been investigated. I put forward the hypothesis that the cotylean sucker is a larval character used for attachment during settlement and metamorphosis, and that it has been retained in the adult as a holdfast. Furthermore, I propose that cotyleans without suckers have lost their suckers because they no longer have a distinct pelagic larval stage. Evidence to support this hypothesis comes from A. acroporae, a sucker-less cotylean that develops larval lobes and ciliary tufts inside the egg capsule but undergoes metamorphosis before hatching . A. acroporae no longer has a free-swimming larval stage and no longer develops a sucker for attachment during settlement and metamorphosis. In order to test this hypothesis, the life history strategies of the seven other cotylean species known to lack ventral suckers  would have to be investigated, and I would predict that they have lost a larval stage either developing directly or via an encapsulated larva. Do the larval stages of acotylean species possess a sucker to attach to the substrate at settlement? This has not been investigated or noted, and is unlikely. Although there are a few acotyleans that have adhesive organs (genital suckers: Leptoplana tremellaris, Itannia ornata; adhesive discs: cestoplanids), all acotylean species known to have a larval stage do not have suckers as adults.
All that we know about polyclad settlement behavior comes from observations of larvae in culture. They become demersal, whirl in circles on the substrate, come to a halt and contract their bodies into various shapes [14, 30]. Some Götte’s larvae become smaller and partially reabsorb their lobes , though this behavior is considered pathological [22, 39], based on the presence of necrotic cells . There are only three reports of larvae settling and undergoing metamorphosis in culture - N. australis, S. uniporus and M. crozieri - but these were casual observations, and the morphology of the settling larvae was not studied in detail. My observations of late-stage larvae collected from the plankton (for example, Figures 3E and 4 in their elongated, dorso-ventrally flattened form with reduced lobes) reveal that their morphology is very plastic. These larvae can crawl on the bottom of a Petri dish, but can also contract, becoming spherical and lobed again before swimming off readily. This ability to alternate between swimming and crawling could allow the larva to sample different substrates before losing the lobes and ciliary band at metamorphosis.
The only data available on morphological changes during metamorphosis come from larvae collected from the plankton [14, 37], and from P. reticulata, which undergoes intra-capsular metamorphosis . In P. reticulata, the lobes, ciliary band and the apical and caudal sensory cilia are lost over 10 days (inside the egg capsule), and the frontal organ is lost shortly after (post hatching) . Ruppert  observed the gradual dwindling, reduction and reabsorption of lobes into the body wall and the incorporation of the prototrochal cells into the juvenile epidermis. Over this time many of the organs that persist in the juvenile and adult develop further, for example, intestinal branches develop, eyes increase in number and the simple larval pharynx becomes a plicate pharynx [6, 11, 37]. The body becomes dorso-ventrally flattened, elongated and thin with prominent growth in the anterior half . Eyes present in the larvae persist into the juvenile and adult, although with modifications. The epidermal eye conceivably sinks into the parenchyma and changes into a cerebral eye with the addition of two ganglionar cells . The cilia of the epithelial pigmented cell persist in the larval cerebral eye, while they disappear in the cerebral eye of the adult . Due to the difficulty of culturing polyclad larvae to settlement and metamorphosis [29, 56], and until larval husbandry techniques improve, species with intermediate development provide a rare opportunity to study polyclad metamorphosis. A. acroporae (the Acropora-eating polyclad) is of particular interest, due to its accessibility (a common pest in reef tanks) and its more typical eight-lobed larval morphology .
Phylogenetic and developmental scenarios on the evolutionary origins of polyclad larval characters
Polyclad larval features bear some similarity to larval characters of parasitic (neodermatan) flatworms and more distantly related spiralians. The first larval stages of neodermatans have, for example, ciliated epidermal cells placed in zones (oncomiracidium larvae, ) or on ridges (Schistosoma mansoni miracidium, ) for swimming to locate a host. They have a sucker (haptor) for attachment during metamorphosis , pigmented and putative unpigmented photoreceptors, and ciliary sensila that are involved in phototaxis, geotaxis and rheotaxis . Ciliary bands for locomotion and feeding and sensory apparatus for orientation in the water column are also found in many spiralian and metazoan larvae. Morphological similarities in these larval features led to the suggestion that they are evolutionarily conserved and that the biphasic life cycle is a shared primitive feature of marine invertebrates [11, 69, 70]. Cell lineage data were seen to support this idea, with polyclad and other spiralian larval ciliary bands considered homologous due to similarities in blastomere contribution . However, recent hypotheses of animal phylogeny do not lend support to this evolutionary scenario, and new data from cell lineage studies are revealing variation in the fate maps of spiralian blastulae [48, 72] that makes comparing cell fates between polyclad and other spiralians difficult.
Our current understanding of animal and flatworm phylogeny (Figure 1A, B [9, 10]) shows that the sister taxa of polyclads are all direct-developing. This suggests that indirect development in polyclads has evolved independently of similar life cycles found in parasitic flatworms and some other spiralian taxa. It follows then that morphological similarities in larval characters between polyclads, neodermatans and other spiralians are likely a result of parallel or convergent evolution. Morphological and molecular comparisons of these larval features may shed light on whether these features represent the redeployment of conserved cell types, or the convergent invention of similar structures. The argument for homology of anatomical structures between polyclads and other spiralians based on similar blastomere origins is problematic because it is difficult to identify the embryonic quadrants in the equally cleaving polyclad embryo. Boyer et al.  identified the injected cells in Hoploplana inquilina retrospectively by comparing their contributions to the formation of larval ectoderm (and other cell fates, such as mesoderm formation), with those of other spiralian cell lineages. For example, as the D quadrant formed the dorsal axis in annelids and molluscs, the blastomere in polyclads that gives rise to dorsal ectoderm was identified as the D quadrant. However, this assumes that there is no variation among spiralian taxa, and as recent lineage studies have highlighted, there is a growing list of cell fate changes between taxa, even among the derivation of the mesoderm, which was once thought to be highly conserved [48, 72]. In light of this variation, homologizing blastomeres - and homologizing structures based on blastomere origins - between the equally cleaving polyclads and other spiralian taxa may not be the most appropriate method for reconstructing the evolution of similar characters.
Marine life histories show tremendous variation with, for example, congeners that have long-lived feeding larvae and direct development [74, 75]. This variation may be strongly influenced by phylogeny, or it may be a result of a species biogeographic distribution and environmental conditions [75, 76]. In order to investigate the evolutionary and biogeographic dynamics of mode of development in polyclads, data are needed on the life history strategies of many more species at different latitudes and regions, and these life history data need to be considered within the framework of a robust molecular phylogeny. As all cotylean species investigated to date develop via an eight-lobed larva, it is probable that this is the ancestral condition for this suborder. However, it is too soon to determine whether similarities in larval structures among (and between) Götte’s and Müller’s larvae, are due to common ancestry or convergence.
A phylogeny of the polyclads, incorporating early branching taxa of both suborders (identifiable from existing hypotheses on polyclad inter-relationships [5, 66, 77]), would reveal the macroevolutionary distribution of developmental modes across families, and would shed light on the ancestral condition for the order. Species-level phylogenies of polyclad clades that show diverse modes of development (for example, the acotylean families: Notoplanidae, Stylochidae, Leptoplanidae) will allow us to investigate the rate and frequency of changes in mode of development. These phylogenies will also allow us to identify sister taxa with different developmental modes, which may be used for comparative studies of the developmental processes involved in the gain and/or loss of larval characters. Finally, understanding the intra-specific variation of larval phenotypes on which contemporary selective pressures and developmental, functional and physiological constraints act, will allow us to gain a better picture of the microevolutionary processes that generate macroevolutionary patterns in mode of development.
There is still much that we do not know about polyclad larval diversity, development and evolution. With the mode of development described for less than 8% of known species, there are undoubtedly more life history strategies and larval morphologies that remain to be discovered. With a greater understanding of the diversity, ecology, evolution and development of polyclad larvae, we will gain insight into the functional significance of variation in larval characters, such as the number of larval lobes and the ciliary band configurations. I have stressed the need for a phylogeny of the polyclads as a framework to understand the distribution of larval features across clades, and to direct research into the morphological and developmental differences between direct and indirect-developing species. In order to examine the turnover of morphological features over ontogeny, we also need to devote time to developing larval culturing techniques, and we must take full advantage of intermediate developing species as models for studies in metamorphosis.
Polyclads offer an excellent system with which to explore the anatomical convergence of larval structures among flatworms and spiralians. Polyclads are the only flatworms from which experimentally accessible embryos can be readily obtained and reared outside of their protective egg capsule (due to techniques pioneered by Rooney and Boyer ), and may therefore provide valuable insight into the evolution of flatworm and spiralian development. Of the few polyclad species that can be collected in large numbers, the cotylean M. crozieri is emerging as a tractable system to study polyclad embryology and larval development [52, 53]. The relative ease of collection in the field and extraction of naked embryos, its comparatively fast development and large egg size make M. crozieri a promising polyclad model species for evolutionary developmental studies. Transcriptomic and/or genomic resources currently available for a number of polyclad and other spiralian taxa are facilitating comparative studies of the molecular mechanisms underlying the development of larval characters. When considered in a phylogenetic framework, these developmental data will shed light on the nature of the evolutionary relationship between spiralian larval characters and their underlying developmental mechanisms.
Most of these images of larvae were taken at the Smithsonian Marine Station at Fort Pierce, USA, while I was a Smithsonian Marine Science Network Postdoctoral Fellow. I thank Drs Mary Rice, Jon Norenburg and Allen Collins and all the staff of the Smithsonian Marine Station for their support during my fellowship. I also thank Professors Brian Hall, Max Telford, Elaine Seaver, Mark Martindale and Dr Andrew Gillis for their support and for discussion of ideas. Finally, I wish to thank two anonymous reviewers for their helpful comments. The author is funded by an NSERC grant awarded to Professor Brian Hall. Smithsonian Marine Station at Fort Pierce, Contribution No. 939.
- Park JK, Kim KH, Kang S, Kim W, Eom KS, Littlewood DTJ: A common origin of complex life cycles in parasitic flatworms: evidence from the complete mitochondrial genome of Microcotyle sebastis (Monogenea: Platyhelminthes). BMC Evol Biol. 2007, 7: 11-10.1186/1471-2148-7-11.PubMed CentralView ArticlePubMedGoogle Scholar
- Larsson K, Jondelius U: Phylogeny of Catenulida and support for Platyhelminthes. Org Divers Evol. 2008, 8: 378-387. 10.1016/j.ode.2008.09.002.View ArticleGoogle Scholar
- Whittington ID, Chisholm LA, Rohde K: The larvae of Monogenea (Platyhelminthes). Adv Parasit. 2000, 44: 139-232.View ArticleGoogle Scholar
- Caira JN, Littlewood DTJ: Diversity of Platyhelminthes. Encyclopedia of Biodiversity: volume 5. Edited by: Levin SA. 2001, New York: Academic Press, 863-899.View ArticleGoogle Scholar
- Rawlinson KA, Gillis JA, Billings RE, Borneman EH: Taxonomy and life history of the acropora-eating polyclad flatworm: Amakusaplana acroporae nov. sp. (Polycladida, Prosthiostomidae). Coral Reefs. 2011, 30: 693-705. 10.1007/s00338-011-0745-3.View ArticleGoogle Scholar
- Kato K: On the development of some Japanese polyclads. Jpn J Zool. 1940, 8: 537-573.Google Scholar
- Poulter JL: Hawaiian polyclads: prosthiostomids I. Pac Sci. 1975, 29: 317-339.Google Scholar
- Reisinger E, Cichocki I, Erlach R, Szyskowitz T: Ontogenetische studien an turbellarien: ein beitrag zur evolution der dotterverarbeitung im ektolecitalen [Article in German]. J Zoolog Syst Evol Res. 1974, 12: 161-195.View ArticleGoogle Scholar
- Giribet G, Dunn CW, Edgecombe GD, Hejnol A, Martindale MQ, Rouse GW: Assembling the spiralian tree of life. Animal evolution: genes, genomes, fossils and trees. Edited by: Telford MJ, Littlewood DT. 2009, Oxford: Oxford University Press, 52-64.View ArticleGoogle Scholar
- Littlewood DTJ: Platyhelminth systematics and the emergence of new characters. Parasite. 2008, 15: 333-341. 10.1051/parasite/2008153333.View ArticlePubMedGoogle Scholar
- Jägersten G: Evolution of the metazoan life cycle: a comprehensive theory. 1972, New York: Academic PressGoogle Scholar
- Rawlinson KA: Biodiversity of coastal polyclad flatworm assemblages in the wider Caribbean. Mar Biol. 2008, 153: 769-778. 10.1007/s00227-007-0845-3.View ArticleGoogle Scholar
- Martín-Durán JM, Egger B: Developmental diversity in free-living flatworms. EvoDevo. 2012, 3: 7-10.1186/2041-9139-3-7.PubMed CentralView ArticlePubMedGoogle Scholar
- Lang A: Die polycladen (seeplanarien) des golfes von neapel und der angrenzenden meeresabschnitte: eine monographie. 1884, Leipzig, Germany: Verlag Wilhelm EngelmannGoogle Scholar
- Rawlinson KA, Bolaños DM, Liana MK, Litvaitis MK: Reproduction, development and parental care in two direct-developing flatworms (Platyhelminthes: Polycladida: Acotylea). J Nat Hist. 2008, 42: 2173-2192. 10.1080/00222930802262758.View ArticleGoogle Scholar
- Rho S: Studies on the polyclad turbellaria of Korea: II spawning and early development of Stylochus ijimai yeri & kaburaki and Pseudostylochus sp. under laboratory conditions. Bull Fish Res Dev Agency. 1976, 15: 123-138.Google Scholar
- Lee K-M, Beal M, Johnston EL: A new predatory flatworm (Platyhelminthes, Polycladida) from Botany Bay, New South Wales, Australia. J Nat Hist. 2006, 39: 3987-3995. 10.1080/00222930500485263.View ArticleGoogle Scholar
- Jennings KA, Newman LJ: Four new stylochid flatworms associated with oysters from southeast Queensland, Australia. Raffles Bull Zool. 1996, 44: 493-508.Google Scholar
- Galleni L: Polyclads of the Tuscan coast. II. Stylochus alexandrinus Steinböck and Stylochus mediterraneus n. sp. from the rocky shores near Pisa and Livorno. Boll Zool. 1976, 43: 139-148. 10.1080/11250007609434892.View ArticleGoogle Scholar
- Gammoudi M, Noreña C, Tekaya S, Prantl V, Egger B: Insemination and embryonic development of some Mediterranean polyclad flatworms. Invertebr Reprod Dev. 2012, 56: 272-286. 10.1080/07924259.2011.611825.View ArticleGoogle Scholar
- Girard C: Researches upon nemerteans and planarians: I, embryonic development of planocera elliptica. 1854, Philadelphia: Merrihew and ThompsonGoogle Scholar
- Hofker J: Faunistische beobachtungen in der zuidersee während der trockenkegung [Article in German]. Z Morph Ökol Tiere. 1930, 18: 202-216.View ArticleGoogle Scholar
- Merory M, Newman LJ: A new stylochid flatworm (Platyhelminthes, Polycladida) from Victoria, Australia and observations on its biology. J Nat Hist. 2005, 39: 2581-2589. 10.1080/00222930500082045.View ArticleGoogle Scholar
- Murina G-V, Grintsov V, Solonchenko A: Stylochus tauricus, a predator of the barnacle balanus improvisus in the black sea. Hydrobiologia. 1995, 305: 101-104. 10.1007/BF00036369.View ArticleGoogle Scholar
- Anderson DT: The embryonic and larval development of the turbellarian Notoplana australis (Schmarda, 1859) (Polycladida: Leptoplanidae). Mar Freshw Res. 1977, 28: 303-310. 10.1071/MF9770303.View ArticleGoogle Scholar
- Surface FA: The early development of a polyclad, Planocera inquilina. Proc Acad Nat Sci Philadelphia. 1907, 59: 514-559.Google Scholar
- Teshirogi W, Ishida S, Jatani K: On the early development of some Japanese polyclads. Rep Fukara Mar Biol Lab. 1981, 9: 2-31.Google Scholar
- Lacalli TC: The nervous system and ciliary band of Müller’s larva. Proc R Soc Lond Ser B-Biol Sci. 1982, 217: 37-58. 10.1098/rspb.1982.0093.View ArticleGoogle Scholar
- Semmler H, Wanninger A: Myogenesis in two polyclad platyhelminths with indirect development, Pseudoceros canadensis and Stylostomum sanjuania. Evol Dev. 2010, 12: 210-221. 10.1111/j.1525-142X.2010.00405.x.View ArticlePubMedGoogle Scholar
- Smith NF, Johnson KB, Young C: Phylum Platyhelminthes. Atlas of marine invertebrate larvae. Edited by: Young CM, Sewell MA, Rice ME. 2002, San Diego: Academic Press, 123-148.Google Scholar
- Selenka E: Zoologische studien: 2 Zur entwickelungsgeschichte der seeplanarien. 1881, Leipzig, Germany: W. EngelmannGoogle Scholar
- Newman LJ, Norenburg JL, Reed S: Taxonomic and biological observations on the tiger flatworm, Maritigrella crozieri (Hyman, 1939), new combination (Platyhelminthes, Polycladida, Euryleptidae) from Florida waters. J Nat Hist. 2000, 34: 799-808. 10.1080/002229300299264.View ArticleGoogle Scholar
- Hallez P: Considerations sur la détermination des plans de seglentation dans l’embryologénie du Leptopana tremellaris. Bull Sci Depart Nord. 1879, 1: 264-266.Google Scholar
- Ching HL: Redescription of Eurylepta leoparda Freeman, 1933 (Turbellaria: Polycladida), a predator of the ascidian Corella willmeriana Herdman, 1898. Can J Zool. 1977, 55: 338-342. 10.1139/z77-045.View ArticleGoogle Scholar
- Van den Biggelaar JAM, Van Loon AE, Damen WGM: Mesentoblast and trochoblast specification in species with spiral cleavage predict their phyletic relations. Nether J Zool. 1996, 46: 8-21.View ArticleGoogle Scholar
- Jokiel PL, Townsley SJ: Biology of the polyclad Prosthiostomum (Prosthiostomum) sp., a new coral parasite from Hawaii. Pac Sci. 1974, 28: 361-373.Google Scholar
- Ruppert EE: A review of metamorphosis of turbellarian larvae. Settlement and metamorphosis of marine invertebrate larvae. Edited by: Chia FS, Rice ME. 1978, New York and Oxford: Elsevier, 65-82.Google Scholar
- Dawydoff C: Les forms larvaires de polyclades et de némertes du plankton indochinois [Article in French]. Bull Biol Fr et Belg. 1940, 74: 1-54.Google Scholar
- Heath H: Sexually mature turbellarian resembling Müller’s larva. J Morphol. 1928, 45: 187-207. 10.1002/jmor.1050450106.View ArticleGoogle Scholar
- Götte A: Abhandlungen zur Entwicklungsgeschichte der Thiere: I. Heft: Untersuchungen zur Entwicklungsgeschichte der Whrmer. 1882, Leipzig: Beschreibender Theil, 1-58.Google Scholar
- Pearse AS: Polyclads of the east coast of North America. Proc US Nat Mus. 1938, 86: 67-98. 10.5479/si.00963801.86-3044.67.View ArticleGoogle Scholar
- Kato K: Platyhelminthes (Class Turbellaria). Invertebrate embryology. Edited by: Kume M, Dan K. 1957, Belgrade: Nolit, 125-142. 1968 translationGoogle Scholar
- Müller J: Über verschiedene formen von Seethieren [Article in German]. Arch Anat Physiol Wiss Med. 1854, 69-98.Google Scholar
- Dalyell C: The powers of the creator displayed in the creation, or observations of life amidst the various forms of the humbler tribes of animated nature. 1853, London: II. J Van Voorst, 95-106.Google Scholar
- Younossi-Hartenstein A, Hartenstein V: The embryonic development of the polyclad flatworm Imogine mcgrathi. Dev Genes Evol. 2000, 210: 383-398. 10.1007/s004270000086.View ArticlePubMedGoogle Scholar
- Bonar DB, Hadfield MG: Metamorphosis of the marine gastropod Phestilla sibogae: I. light and electron microscopic analysis of larval and metamorphic stages. J Exp Mar Biol Ecol. 1974, 16: 1-29. 10.1016/0022-0981(74)90068-9.View ArticleGoogle Scholar
- Page LR: Molluscan larvae: pelagic juveniles or slowly metamorphosing larvae?. Biol Bull. 2009, 216: 216-225.PubMedGoogle Scholar
- Meyer NP, Boyle MJ, Martindale MQ, Seaver EC: A comprehensive fate map by intracellular injection of identified blastomeres in the marine polychaete Capitella teleta. EvoDevo. 2010, 1: 8-10.1186/2041-9139-1-8.PubMed CentralView ArticlePubMedGoogle Scholar
- Yamaguchi E, Seaver EC: The importance of larval eyes in the polychaete Capitella teleta: effects of larval eye deletion on formation of the adult eye. Invertebr Biol. 2013, 132: 352-367. 10.1111/ivb.12034.View ArticleGoogle Scholar
- Reiter D, Boyer B, Ladurner P, Mair G, Salvenmoser W, Rieger R: Differentiation of the body wall musculature in Macrostomum hystricinum marinum and Hoploplana inquilina (Plathelminthes), as models for muscle development in lower Spiralia. Roux’s Arch Dev Biol. 1996, 205: 410-423. 10.1007/BF00377221.View ArticleGoogle Scholar
- Bolaños DM, Litvaitis M: Embryonic muscle development in direct and indirect developing marine flatworms (Platyhelminthes, Polycladida). Evol Dev. 2009, 11: 290-301. 10.1111/j.1525-142X.2009.00331.x.View ArticlePubMedGoogle Scholar
- Rawlinson KA: Embryonic and post-embryonic development of the polyclad flatworm Maritigrella crozieri: implications for the evolution of spiralian life history traits. Front Zool. 2010, 7: 12-10.1186/1742-9994-7-12.PubMed CentralView ArticlePubMedGoogle Scholar
- Lapraz F, Rawlinson KA, Girstmair J, Tomiczek B, Berger J, Jékely G, Telford M, Egger B: Put a tiger in your tank: the polyclad flatworm Maritigrella crozieri as a proposed model for evo-devo. EvoDevo. 2013, 4: 29-10.1186/2041-9139-4-29.PubMed CentralView ArticlePubMedGoogle Scholar
- Lacalli TC: The suboral complex in the Müller’s larva of Pseudoceros canadensis (Platyhelminthes, Polycladida). Can J Zool. 1988, 66: 1893-1895. 10.1139/z88-273.View ArticleGoogle Scholar
- Knight-Jones EW: Relations between metachronism and the direction of ciliary beat in Metazoa. Q J Microsc Sci. 1954, 95: 503-521.Google Scholar
- Scarpa J, Weis B, Ruppert E, Frick J, Ford A, Wright A: Direct evidence for planktotrophy in muller’s larva larva of the tiger flatworm, Pseudoceros crozieri [abstract]. Am Zool. 1996, 36: 107-Google Scholar
- Emlet RB: Functional constraints on the evolution of larval forms of marine invertebrates: experimental and comparative evidence. Amer Zool. 1991, 31: 707-725.View ArticleGoogle Scholar
- Lacalli TC: The brain and central nervous system of Müller’s larva. Can J Zool. 1983, 61: 39-51. 10.1139/z83-004.View ArticleGoogle Scholar
- Lanfranchi A, Bedini C, Ferrero E: The ultrastructure of the eyes in larval and adult polyclads (Turbellaria). Hydrobiologia. 1981, 84: 267-275. 10.1007/BF00026189.View ArticleGoogle Scholar
- Johnson KB, Forward RB: Larval photoresponses of the polyclad flatworm Maritigrella crozieri (Platyhelminthes, polycladida) (Hyman). J Exp Mar Biol Ecol. 2003, 282: 103-112. 10.1016/S0022-0981(02)00448-3.View ArticleGoogle Scholar
- Strathmann RR: Feeding and non-feeding larval development and life history evolution in marine invertebrates. Ann Rev Ecol Syst. 1985, 16: 339-361. 10.1146/annurev.es.16.110185.002011.View ArticleGoogle Scholar
- Anderson DT: The comparative embryology of the polychaeta. Acta Zool. 1966, 47: 1-42. 10.1111/j.1463-6395.1966.tb00738.x.View ArticleGoogle Scholar
- Arenas-Mena C, Suk-YingWong K, Arandi N: Histone H2A.Z expression in two indirectly developing marine invertebrates correlates with undifferentiated and multipotent cells. Evol Dev. 2007, 9: 231-243. 10.1111/j.1525-142X.2007.00155.x.View ArticlePubMedGoogle Scholar
- Hadfield MG, Carpizo-Ituarte EJ, del Carmen K, Nedved BT: Metamorphic competence, a major adaptive convergence in marine invertebrate larvae. Am Zool. 2001, 41: 1123-1131. 10.1668/0003-1569(2001)041[1123:MCAMAC]2.0.CO;2.Google Scholar
- Prudhoe S: A monograph on polyclad turbellaria. 1985, London: British Museum (Natural History)Google Scholar
- Rawlinson KA, Litvaitis MK: Cotylea (Platyhelminthes, Polycladida): a cladistic analysis of morphology. Invertebr Biol. 2008, 127: 121-138. 10.1111/j.1744-7410.2007.00119.x.View ArticleGoogle Scholar
- Collins JJ, King RS, Cogswell A, Williams DL, Newmark PA: An atlas for Schistosoma mansoni organs and life-cycle stages using cell type-specific markers and confocal microscopy. PLoS Negl Trop Dis. 2011, 5: e1009-10.1371/journal.pntd.0001009.PubMed CentralView ArticlePubMedGoogle Scholar
- Jennings JB: Nutritional and respirational pathways to parasitism exemplified in the turbellaria. Int J Parasitol. 1997, 21: 679-691.View ArticleGoogle Scholar
- Haeckel E: The gastrea-theory, the phylogenetic classification of the animal kingdom and the homology of the germ-lamellae. Q J Microsc Sci. 1874, 14: 142-165.Google Scholar
- Hatschek B: Studien über die Entwicklungsgeschichte der Anneliden [Article in German]. Ein Beitrag zur Morphologie der Bilaterien. Arb Zool Inst Univ Wien. 1878, 1: 277-404.Google Scholar
- Nielsen C: Trochophora larvae: cell-lineages, ciliary bands and body regions: 2. other groups and general discussion. J Exp Zool B Mol Dev Evol. 2005, 304B: 401-447. 10.1002/jez.b.21050.View ArticleGoogle Scholar
- Gline SE, Nakamoto A, Cho SJ, Chi C, Weisblat DA: Lineage analysis of micromere 4d, a super-phylotypic cell for Lophotrochozoa, in the leech Helobdella and the sludgeworm Tubifex. Dev Biol. 2011, 353: 120-133. 10.1016/j.ydbio.2011.01.031.PubMed CentralView ArticlePubMedGoogle Scholar
- Boyer BC, Henry JQ, Martindale MQ: Dual origins of mesoderm in a basal spiralian: cell lineage analyses in the polyclad turbellarian Hoploplana inquilina. Dev Biol. 1996, 179: 329-338. 10.1006/dbio.1996.0264.View ArticlePubMedGoogle Scholar
- Byrne M: Life-history diversity and evolution in the Asterinidae. Integr Comp Biol. 2006, 46: 243-254. 10.1093/icb/icj033.View ArticlePubMedGoogle Scholar
- Collin R: Phylogenetic effects, the loss of complex characters, and the evolution of development in calyptraeid gastropods. Evolution. 2004, 58: 1488-1502.View ArticlePubMedGoogle Scholar
- Marshall DJ, Krug PJ, Kupriyanova EK, Byrne M, Emlet RB: The biogeography of marine invertebrate life histories. Annu Rev Ecol Evol Syst. 2012, 43: 97-114. 10.1146/annurev-ecolsys-102710-145004.View ArticleGoogle Scholar
- Faubel A: The Polycladida, Turbellaria: proposal and establishment of a new system. Part I. The Acotylea. Mitteilungen Aus Dem Hambg Zool Mus Inst. 1983, 80: 17-121.Google Scholar
- Rooney LM, Boyer BC: New aspects of development of the polyclad, Hoploplana inquilina. Biol Bull. 1984, 167: 519-520.Google Scholar
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