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Figure 4 | EvoDevo

Figure 4

From: Gene expression suggests conserved aspects of Hox gene regulation in arthropods and provides additional support for monophyletic Myriapoda

Figure 4

Detection of complementary expression patterns of Ubx and aUbx in Glomeris marginata. (A) Stage 2 embryo stained for aUbx. Asterisk marks space free from transcripts. Arrows point to expression in (B); arrowhead points to expression in the anal valves. (A') DAPI counterstaining of the embryo shown in (A). (B) Stage 2 embryo stained for Ubx. Asterisk, arrows and arrowhead are in similar positions to (A). (C) Double staining with aUbx (orange) and Ubx (purple). Asterisk, arrows and arrowhead in similar positions to (A,B). (B',C') Cut out of embryos shown in (B,C) next to each other for ease of comparison of expression pattern. Asterisks, arrows and arrowheads are in similar positions (orange symbols in (C') point to expression of aUbx). (D) Stage 4.1 embryos expressing aUbx (upper) and Ubx (lower) respectively. (E) Posterior part of stage 3 embryo double-stained for aUbx (purple) and Ubx (orange). Arrowheads mark expression of aUbx in dorsal tissue. White arrows point to expression of aUbx in the tips of the legs. Note that aUbx is only at their base. Black arrows indicate segmental expression of aUbx. Asterisk indicates anal valves. (F) Embryo of same stage as in (E) expressing Ubx, with arrows and asterisk in similar positions.

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