Figure 5

Gene expression in semithin sectioned larvae. (A) BnHox4 expression shown in lateral section and (B) magnified area of the abapical part of the larva showing the expression in the cell soma of the wall and roof cells of the internal sac. Expression in the cytoplasma of wall cells is indicated in some areas (arrows). Faint expression is present in the glandular area (gl) of the pyriform organ (asterisk). The nuclei of the wall cells are brightly stained with basic fuchsine (arrow heads). (C) BnSoxB2 expression is visible in the epidermal and mesodermal blastemas and wall cells. (D) Detail of the apical disc showing expression of BnSoxE in cells of the mesodermal blastema (two cells lacking expression are indicated by arrows). The epidermal blastema lacks expression (some basally lying nuclei of the cells of this layer are indicated by arrow heads). All sections are additionally stained with basic fuchsine (pink). co, corona; eb, epidermal blastema; pe, pallial epithelium; mb, mesodermal blastema; n, neck; r, roof; w, wall; y, yolk inclusion. Scale bars in (A, C) 50 μm; (B, D) 25 μm.