Figure 3

Double staining of Glomeris PRGs (purple) with engrailed (red). Pictures denoted in capital letters are whole mounts; pictures denoted in small letters show close-ups. All embryos are flat-mounted; yolk removed. (A, B) engrailed (en) is expressed anterior adjacent to even-skipped (eve). Arrow in (B) points to dot-like expression in the CNS. (C, D) en is expressed anterior adjacent to runt (run). Asterisks in (D) denote enhanced dorsal expression in the postmaxillary segment. (E, F) en is expressed posteriorly adjacent to opa. The arrows mark opa expression at the position where the premandibular and postmaxillary appendages should form, but are repressed by unknown mechanisms. (G, H) en is expressed posterior to sloppy-paired (slp) at the anterior edge of the SAZ. (I, J) en is expressed posterior to and partially overlapping with pairberry-1 (pby-1) in nascent segments. Abbreviations: md, mandibular segment; SAZ, segment addition zone; st, stage; T, trunk segment.