Figure 5
From: The Pax gene eyegone facilitates repression of eye development in Tribolium
Effect of postembryonic eyg knockdown on adult eye morphology in Tribolium. (a-f) Scanning electron microscopy images of adult head morphology. Scale bars corresponds to 100 μm. (a and b) Dorsal view of wild type (a) and eyg knockdown head (b). (c and d) Lateral view of wild type (c) and eyg knockdown head (d). (e and f) High magnification view of compound eye in wild type (e) and eyg knockdown (f) specimen. (e) Small arrowheads point at interommatidial bristles in the anterior dorsal and ventral regions of the compound eye where no bristles are formed in wild type animals. Large arrowhead points at irregularly shaped facet. Open arrowhead indicates multiplied interommatidial bristles. Arrow points at indenture at the tip of the extended gena. (g) Bar graphs showing comparison of compound eye size measured in average number of ommatidia of untreated and experimental animals injected with different dsRNA preparations and at different time points as indicated. Error bars represent standard deviation. Unlinked single asterisks indicate significant difference to EGFP dsRNA injected control based on two-tailed t-test (P < 0.05). Unlinked double asterisk indicates highly significant difference to untreated control based on two-tailed t-test (P < 0.005). Linked double asterisk indicates highly significant difference between EGFP dsRNA injected control and eyg dsRNA injected animals injected during early larval development based on two-tailed t-test (P < 0.005).