Figure 2

AmqDelta1 developmental expression. (A) Expression of AmqDelta1 is first detected in cells in the outer layer in early cloud stage embryos (A’). (B) Expression remains in scattered cells (B’) in the outer layer, more densely occurring at the boundary between the two layers. (C) Late spot stage embryos express AmqDelta1 in the outer layer, as well as in cells under the center of the forming pigment ring (white arrow) (C’) and at the anterior pole (arrowhead) (C”). (D) Ring stage embryos display expression in the forming middle layer (white arrowhead), as well as the anterior pole (inset). (E) In late ring embryos, expression is detected in the subepithelial (middle) layer (white arrowhead), and in the globular cells (double arrowhead) that are migrating from the subepithelial layer to the outer margin (E’). (F) Expression in larvae persists in the globular cells (double arrowhead), now located around the outer margin (F’,F”), and within the pigment ring (white arrow). All panels display cleared, whole mount embryos, except (F”) in which the embryo was sectioned after staining. (C”), anterior view; (C’), posterior view; all remaining panels are lateral views with posterior to the top.