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Figure 4 | EvoDevo

Figure 4

From: Development of an embryonic skeletogenic mesenchyme lineage in a sea cucumber reveals the trajectory of change for the evolution of novel structures in echinoderms

Figure 4

The skeletogenic centers of sea cucumbers do not express gata4/5/6 , gata1/2/3 , tbr , tgif or foxn2/3. WMISH performed against gastrula stage embryos (A-F) show localization of only PpErg and PpAlx1 transcripts in the skeletogenic mesenchyme (A). A probe designed against PpGata4/5/6 is localized to other mesenchyme cells (B). No other factor identified in this study showed expression in mesenchyme. WMISH with probes antisense to PpGata1/2/3 and PpTbr are showed localized staining at the tip of the archenteron in gastrulae (C, D), while PpFoxn2/3 antisense probes stain only within the ectoderm (E) and those antisense to PpTgif in the endoderm (F). In larvae, PpErg antisense probes are localized to the SM (G); and see higher magnification in inset. Probes against PpGata4/5/6 stain within the midgut (H), while those against PcGata1/2/3 (I) are present in the posterior coelom. No staining is found for PpTbr at this stage (J), see higher magnification of SM cells in insert that show no staining. Probes against PpFoxn2/3 (K) ultimately show localization in the posterior foregut. PpTgif antisense probes are localized to the midgut of larvae (L).

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ISSN: 2041-9139