Figure 6

In situ hybridization of B-class genes in Commelina communis and C. dianthifolia. (A-C) C. communis flower sections showing expression of GLOBOSA (GLO) in early to late stages of inner tepal and stamen/staminode development, and late stages of gynoecial development. (D-F) Mid-stage C. communis flowers sectioned longitudinally showing DEFICIENS (DEF) expression in the dorsal inner tepals, dorsal staminodes, and ventral stamens. No transcripts were detected in the outer tepals, ventral inner tepals, or gynoecium. (G) Late-stage longitudinal section of a C. communis flower showing DEF expression in the dorsal inner tepals and staminodes. (H-I) Transverse sections through late-stage C. communis flowers showing DEF expression in the dorsal inner tepals, dorsal staminodes, and ventral stamens. (J) Early-stage C. dianthifolia flower showing comparable DEF expression in the dorsal and ventral regions of the stamen primordia. (K-M) Mid-stage C. dianthifolia flowers showing DEF expression in the dorsal inner tepals, dorsal staminodes, ventral inner tepals, and ventral staminodes. (N) Late-stage C. dianthifolia flower showing DEF expression in all inner tepals and the dorsal staminodes. (O) Sense DEF control in C. communis shows little to no staining. fm, floral meristem; ds, dorsal outer tepal; vs, ventral stamen; dp, dorsal inner tepal; vp, ventral inner tepal; da, dorsal androecium; va, ventral androecium; gy, gynoecium; sty, style; ov, ovary.