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Figure 3 | EvoDevo

Figure 3

From: Hidden in the crowd: primordial germ cells and somatic stem cells in the mesodermal posterior growth zone of the polychaete Platynereis dumerillii are two distinct cell populations

Figure 3

The PGCs arise between 3-8 hpf. Embryos were incubated for different periods in EdU and the developing young worms were fixed for Vasa and EdU detection at at 7 dpf. (A) Neither PGCs nor the MPGZ have incorporated EdU between 1 and 3 hpf. (B) Nuclei of the PGCs, but not of the MPGZ, are labeled at 3-7 hpf. (C) When embryos were incubated from 7-9 hpf, only two out of four PGCs are labeled. (D) No labeled PGCs are detectable, when embryos were incubated later than 8 hpf. Asterisks: PGCs, red: incorporation of EdU, arrows: MPGZ, green: Vasa immunohistochemistry as a marker for PGCs and the MPGZ. A-D are overlayed stacks of confocal images. A'-D' show the PGCs at higher magnification. A"-D" are schematic representations. The three larval segments are labeled C = cephalic segment, I, and II. Anterior of the larvae is to the top. Scale bar = 50 μm in A-D and 20 μm in A'-D'.

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ISSN: 2041-9139