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Figure 7 | EvoDevo

Figure 7

From: Developmental coupling of larval and adult stages in a complex life cycle: insights from limb regeneration in the flour beetle, Tribolium castaneum

Figure 7

Knockdown verification of double-stranded RNA (dsRNA)-injected larvae using semiquantitative polymerase chain reaction. Day-0 sixth-instar larvae were injected with 2 μg/μL ampicillin-resistance (ampr), dachshund (dac), Distal-less (Dll), abrupt (ab) and spineless (ss) dsRNA, and total RNA was isolated from day-2 seventh-instar larvae. This RNA was converted to cDNA, and gene-specific primers were used to amplify the gene products. ampr dsRNA-injected larvae served as controls. The cycle numbers were as follows: ab = 38; dac = 42; Dll = 39; ss = 40; rp49 = 34.

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ISSN: 2041-9139