Figure 2

Duplex PCR assay for sex determination in Strigamia maritima. (A) Positive control. Sex-determination assay on adults of known sex. Two males, M1 and M2 generate PCR bands at 398 bp and 288 bp corresponding to the autosomal band and Y chromosome band, respectively. Two females, F1 and F2 generate a single band at 398 bp corresponding to the autosomal band. (B) Experimental assay. Sex determination of single embryos of unknown sex. Lanes 1, 2, 3, 6 and 7 generate single PCR bands (398 bp) and so are inferred to contain DNA derived from female embryos. Lanes 4 and 5 generate two PCR bands (398 bp, 288 bp) and so are inferred to be from males. NTC, no template control (generates no bands). The sizes of a 1 kb (L1) and 100 bp (L2) DNA ladder are shown.