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Figure 2 | EvoDevo

Figure 2

From: Identification of a putative germ plasm in the amphipod Parhyale hawaiensis

Figure 2

One-cell Parhyale embryos have a distinct cortical cytoplasmic region that corresponds to the RCB. (A) Following fertilization, a diffuse white spot that is more refractive than the surrounding cytoplasm appears at the cortex of the one-cell embryo (arrow). (B) The cortical cytoplasmic domain develops a ring-like appearance visible under incident illumination, with a yolk-free cytoplasmic area surrounded by small granules. (C) vasa transcripts are localized to the RCB, which corresponds to the distinct cortical cytoplasmic domain in (A). (D) A lateral view of vasa staining shows the concave shape of the cortex at the position of the RCB and the cone-shaped morphology of the RCB itself (arrowhead). (E) The nucleic acid dye SYTOX Green weakly labels the RCB (arrow) and strongly labels the polar bodies (arrowhead) showing that the RCB can first be detected on the vegetal side of the embryo, opposite the polar bodies. Scale bar: (B) 50 μm (C) 25 μm (D) 50 μm (E) 100 μm. RCB, RNA-containing body.

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