Figure 10

Effect of X-ray irradiation and hydroxyurea (HU) treatment. (A) Number of EdU + cells per area at different times after X-ray irradiation (150 Gy) and in unirradiated controls, after a five hour 50 μm EdU pulse (average and standard deviations of 7 to 14 vesicles per time point). (B) Number of EdU + cells per area in vesicles treated with HU (40 mM for seven days) and in non-treated controls (all vesicles were allowed to recover for 24 hours in HU-free medium, followed by a five hour 50 μm EdU pulse; average and standard deviation of seven independent experiments). (C) Number of AcTub + cells per area in vesicles treated with HU (40 mM for seven days) and in non-treated controls (average and standard deviation of nine to eleven vesicles pooled from three independent experiments). (D) WMISH of em-muc-1 in a vesicle treated with HU (40 mM for seven days) and in a non-treated control. *P < 0.001,**P < 0.01. The Mann–Whitney U-test was used for A-C.