WMISH detection of em-nos-1 (A-C) and em-nos-2 (D-I). A and D, general view and B and E, detail of the positive cells in the germinative layer. C and F, co-localization in the germinal layer of em-nos-1 and em-nos-2 (green) with EdU incorporation (red) after a five hour 50 μM pulse. Double positive cells are indicated with a filled arrowhead, whereas cells expressing a nanos gene but EdU − are indicated with an open arrowhead. G, Expression of em-nos-2 in cells surrounding a brood capsule bud (dashed circle). H, Protoscolex bud. I, Later protoscolex development. Arrows indicate em-nos-2+ cells in the position of the developing lateral ganglia. Abbreviations are as in Figure 2. Bars represent 10 μm except for H, 20 μm.