Am-eve expression in dsRNA knockdown embryos. (A and B) Am-eve expression at stages 3–4. Am-eve stripe expression starts at the anterior to posterior fashion. Stripes begin to split (arrow) and become distinguished just prior to gastrulation (B). (C) Am-eve mRNA is detected throughout stage 4 embryos following knockdown of Am-Toll. The strongest expression is found at the anterior and posterior ends of the embryo, and some stripe expression can be discerned (arrow). (D) In Am-DppRNAi embryos only the first three stripes (1–3) of Am-eve expression is seen. The posterior half of the embryo Am-eve mRNA appears as one broad domain of expression. (E) Am-eve transcripts are expressed in stripes after Am-GB19066RNAi but are much less distinct, particularly throughout the posterior half of the embryo. The gastrulation ventral furrow appears expanded and there is some disruption of Am-eve stripes across this region (white arrow). F) Schematic representation of WT embryos at stage 9 with segments labeled. (G) Representation of the Am-Dp pRNAi phenotype showing loss of most segments and amnion. (H) Summary schematic of Am-TollRNAi embryos labeling remaining segmens present in these embryos. (I) Representation of Am-GB19066RNAi labeling remaining segments present in RNAi treated embryos. Abbreviations: amnion (am), procephalic lobe (PC), mandible (mb), maxillae (mx), thorax segment (T1-3), abdominal segment (A1-9), terminal segment (T), antenna (ant), labrum (lb), serosa (SR).