Figure 6

sox2 knockdown. (A- C) Effects of sox2 MO on eye development at 42 hr post fertilization (hpf). Sections of uninjected (A), sox2 MO injected (B), and control MO-injected (C) embryos show reduced eye size after sox2 knockdown. Scale bar in A is 150 μm: A-C are the same magnifications. (D- H) sox2 knockdown abolishes lens αA-crys gene expression. In situ hybridization of uninjected (D), sox2 MO-injected (E), and control MO-injected (F) embryos shows no lens αA-crys expression after sox2 knockdown. Sections through the eyes of in situ hybridized uninjected (G) and sox2 injected (G) embryos confirm the absence of αA-crys expression in the sox2 morphant lens. R: retina. L: lens. (I) In situ hybridization showing lens mip gene expression in sox2 MO-injected embryos. (J -L) Effects of sox2 gene knockdown on apoptosis at 42 hpf. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assays of un-injected (J), sox2 MO-injected (K), and control MO injected (L) embryos show apoptosis in the lens (arrow labeled L) and retina (arrow labeled R) in sox2 morphants. Embryos are shown at 42 (A- I) and 48 (J- L) hpf. Scale bars in D, G, and J are 100 μm; magnification is the same in A-C; D-F, I; G and H; and J-L. M. Histogram showing apoptotic cells in the lens of uninjected embryos (blue) and sox2 MO-injected embryos (red). Error bars represent SD. Numbers at the base of the histogram represent sample sizes. P = 0.00, one-way analysis of variance (ANOVA).