Figure 5

DAPT treatment increases NvashA expression in the planula larva. (A-C) Forty-eight hours post fertilization (hpf) animals either treated with control DMSO (A) or with DAPT (B-C). (A) NvashA expression in control animals is detected in the developing pharynx (arrow), in the endoderm (arrowhead), and in the ectoderm (inset). (B) Treatment with DAPT increases NvashA expression in each tissue. (C) Quantitative (q)PCR analysis reveals a three-fold increase in the relative levels of NvashA in DAPT-treated animals. (D-F) Seventy-two hpf animals either treated with control DMSO (D) or with DAPT (E-F). (D) NvashA expression in control animals is detected in the developing pharynx (arrow) and in the endoderm. (E) Treatment with DAPT increases NvashA expression in each tissue. (F) qPCR analysis reveals a three-fold increase in the relative levels of NvashA in DAPT-treated animals. The key in (C) and (F) shows that animals were grown in normal 1/3X sea water (black line between time intervals) or in the presence of DAPT (red line between time intervals). Animals in (A,B,D,E) were quantified into phenotypic classes based on having no, weak, wild-type-like, or strong expression levels. The key is shown in the image and bars at the base of each image represent the percentage of animals in each phenotypic class. Red box in (C and F) indicates the region between 0 and 1.5-fold change, which we consider to indicate no change in expression. All animals are shown in a lateral view with the oral side to the left.