Figure 2

Intact animals of Monocelis sp. (A) Differential interference contrast image. The distribution of the adhesive system is indicated by black arrowheads. (B) Ventral, (C) central and (D) dorsal views of phalloidin-stained actin filaments. White arrow in (C) points at a forked muscle fibre, white arrowheads demarcate the position of the caudal nerve loop. Inset shows a magnified paracnid-like cell. (E) Ventral, (F) central and (G) dorsal view of the serotonergic nervous system. White arrows in (F-G) indicate female pore-related gland cells; white arrowheads indicate gut-related gland cells; hollow arrowheads indicate seminal vesicle-related gland cells. Asterisks in G mark the anterior end of dorsal nerve cords. (H) 30 min BrdU-pulse-labelled S-phase cells (green) and mitoses (red); inset with presumptive testis encircled. ad, adhesive system; alc, anterior lateral nerve cords; an, anterior nerves; br, brain; dc, dorsal nerve cords; fp, female pore; g, gut; ge, germaria; lc, lateral nerve cords; m, mouth; mc, main nerve cords; mp, male pore; ph, pharynx; phl, pharynx lumen; phs, pharynx sheath; phsl, pharynx sheath lumen; ple, plexus; s, statocyst; sty, stylet; sv, seminal vesicle; vc, ventral nerve cords; vp, vaginal pore. Anterior is left in all panels. Scale bar, 100 μm; inset, 25 μm.