|
dsRNA (μg/μl)
|
Embryonic phenotypes (%)
|
n
|
|---|
| |
No germ rudiment*
|
Embryo anteriorly localized**
|
Embryo randomly localized
|
Embryo posteriorly localized
| |
|---|
|
Rp-dl (0.5) stages 2-4
|
44
|
50
|
5
|
0
|
18
|
|
Rp-dl (1.0) stages 2-4
|
32
|
52
|
4
|
12
|
25
|
|
Rp-dl (0.5) stages 5-10
|
22
|
68
|
7
|
3
|
59
|
|
Rp-Toll (0.5) stages 2-10
|
7
|
23
|
30
|
40
|
30
|
|
control (1.0) stages 2-10
|
6
|
-
|
-
|
94
|
50
|
- *“No germ rudiment” phenotype includes empty eggs and halted embryos that do not form a germ rudiment. **At stage 2 embryos anteriorly localized display embryonic rudiment perpendicular to long axis of egg; after stage 5 anteriorly localized embryos are short, with absent or small appendage-like structures (25% penetrant appendage-like phenotype).
