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Table 4 Embryonic phenotypes resulting from Rp-dl pRNAi

From: Toll signals regulate dorsal–ventral patterning and anterior–posterior placement of the embryo in the hemipteran Rhodnius prolixus

dsRNA (μg/μl) Embryonic phenotypes (%) n
  No germ rudiment* Embryo anteriorly localized** Embryo randomly localized Embryo posteriorly localized  
Rp-dl (0.5) stages 2-4 44 50 5 0 18
Rp-dl (1.0) stages 2-4 32 52 4 12 25
Rp-dl (0.5) stages 5-10 22 68 7 3 59
Rp-Toll (0.5) stages 2-10 7 23 30 40 30
control (1.0) stages 2-10 6 - - 94 50
  1. *“No germ rudiment” phenotype includes empty eggs and halted embryos that do not form a germ rudiment. **At stage 2 embryos anteriorly localized display embryonic rudiment perpendicular to long axis of egg; after stage 5 anteriorly localized embryos are short, with absent or small appendage-like structures (25% penetrant appendage-like phenotype).