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Table 4 Embryonic phenotypes resulting from Rp-dl pRNAi

From: Toll signals regulate dorsal–ventral patterning and anterior–posterior placement of the embryo in the hemipteran Rhodnius prolixus

dsRNA (μg/μl)

Embryonic phenotypes (%)

n

 

No germ rudiment*

Embryo anteriorly localized**

Embryo randomly localized

Embryo posteriorly localized

 

Rp-dl (0.5) stages 2-4

44

50

5

0

18

Rp-dl (1.0) stages 2-4

32

52

4

12

25

Rp-dl (0.5) stages 5-10

22

68

7

3

59

Rp-Toll (0.5) stages 2-10

7

23

30

40

30

control (1.0) stages 2-10

6

-

-

94

50

  1. *“No germ rudiment” phenotype includes empty eggs and halted embryos that do not form a germ rudiment. **At stage 2 embryos anteriorly localized display embryonic rudiment perpendicular to long axis of egg; after stage 5 anteriorly localized embryos are short, with absent or small appendage-like structures (25% penetrant appendage-like phenotype).
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