Figure 2

Immunolabeling of larvae of Gymnomenia pellucida. Maximum intensity projections of confocal image stacks; apical is up and scale bar equals 40 μm in all panels. A: Labeling of acetylated α-tubulin-like immunoreactive (−LIR) components; 1 to 2 days posthatching (dph) larva showing abapical neurogenic domain (asterisk) with an outgrowing pair of neurite bundles (arrows). B: Labeling of acetylated α-tubulin-LIR (blue) and FMRF-amide-LIR (yellow) components; 2 to 3 dph larva showing a pair of abapically outgrowing neurite bundles (arrows) as well as a second pair of neurite bundles (double arrowheads) growing out from the neuropil of the apical organ. C: Labeling of FMRF-amide-LIR components; 2 to 3 dph larva. D: Labeling of acetylated α-tubulin-LIR (blue) and FMRF-amide-LIR (yellow) components; 6 to 7 dph larva scanned in ventral aspect showing lateral neurite bundles (arrows) with neurite (arrowhead) branching off to the lateral part of the apical region; note that the neurite bundle on the right side of the animal is not yet fully developed. Abbreviations: ao, apical organ; np, neuropil of apical organ; pt, prototroch; src, suprarectal commissure; tt, telotroch.