|
Early cleavage
|
3 hpf
|
3-5
|
EC
|
1-4 blastomeres
|
100
|
3
|
|
Early hollow blastula
|
1 dpf
|
12
|
EHB
|
Presence of a segmentation cavity containing blastomeres and covered by enveloping layer cells
|
100
|
4
|
|
50% epiboly
|
2 dpf
|
17
|
50% EP
|
Half of the yolk surface covered by periblast and enveloping layer. In between the two layers are embryonic blastomeres that have become ameboid and migrated away from the central blastula
|
100
|
4
|
|
Dispersed blastomere phases
|
4 dpf
|
20-21
|
DBP
|
Yolk surface completely covered by periblast, enveloping layer, and randomly distributed embryonic blastomeres
|
100
|
4
|
|
Reaggregation phases
|
8 dpf
|
22-26
|
RP
|
Embryonic blastomeres remain distributed across yolk, but a subpopulation are beginning to migrate towards a small area of the yolk where the future embryo will form. No discernable embryonic axis present
|
100
|
4
|
|
Solid neural keel
|
10 dpf
|
28
|
SNK
|
Presence of a solid neural keel, head fold, and Kupffer’s vesicle. No somites present
|
50
|
4
|
|
Diapause II
|
32 dpf
|
33
|
DII
|
Presence of optic cups and associated lenses, otic vesicles, functional heart, and 38 to 40 pairs of somites. Heart rate of 0 to 10 bpm
|
100
|
3
|
|
Three-quarter overgrowth
|
9 dpd
|
39
|
3/4 OG
|
Embryo occupies about three-fourths of the perimeter of the yolk. Eyes are heavily pigmented with gold colored material. Presence of incompletely developed gut, liver, and swimbladder
|
40
|
4
|
|
Diapause III
|
24 dpd
|
43
|
DIII
|
Fully formed larva that has completed embryonic development, but has not yet hatched
|
40
|
4
|
|
Adult liver
|
Adult
|
N/A
|
Liver
|
Whole liver from an adult female
|
1
|
3
|
