In vivo cortical localization of NvPar-3::mVenus and NvLgl::mCherry during different stages of N. vectensis development. z-stack sections from the stages are shown in Figure 12. NvPar-3::mVenus (A) and NvLgl::mCherry (B) distributed in different regions inside the cells (C) below the cortical actin labeled with Lifeact::mTq2 (D): NvPar-3::mVenus was always observed towards the apico-lateral membrane while NvLgl::mCherry moved basolaterally. NvPar-3::mVenus is localized at the cell-cell contacts where the separation between apical and basolateral regions is clearly defined (C, gastrula insets). Insets show the asymmetric distribution of NvPar-3::mVenus and NvLgl::mCherry in the cells of ectodermal epithelium at the gastrula stage. The magnified region was selected to represent the asymmetric distribution of those proteins and is not intended for function implication.