Figure 2

Identification of mRNA distribution by whole-mount in situ hybridization. Maternal mRNA of NvaPKC, NvPar-3, NvPar-6, NvPar-1, and NvLgl are asymmetrically distributed during the early development of N. vectensis. In blastula and gastrula stages, these genes are uniformly distributed in the ectoderm. The distribution of all five genes is not consistent during early stages, suggesting no association with the animal-vegetal axis of the embryo; therefore, the protein localization is required.