Two phases of N. vectensis Lgl (NvLgl) and Par-1 (NvPar-1) localization. No asymmetric localization of NvLgl (A) and NvPar-1 (B) occurs during cleavage stages; however, they localize asymmetrically in the epithelial cells at later stages (insets on blastula and gastrula). Similar to some bilaterians, NvLgl and NvPar-1 extend throughout basolateral cortex of the cell. Both proteins distribute throughout the microtubule cytoskeleton (labeled with tubulin antibody staining), and no labeling was observed in the endoderm/gastrodermis (gastrula and polyps were observed). White arrows indicate astral/centrosomal and (pro)nuclear structures. White arrowheads indicate the endodermal layer in gastrula and polyp stages. Actin cytoskeleton is shown as gray. Animal/vegetal axis (A/V) and apical/basal axis are indicated. Insets shown in blastula, gastrula, and polyp stages are higher magnification of the zone indicated with the dashed lines. Images ranging from unfertilized to gastrula and insets in polyp stages correspond to a single slice from z-stack series. Image of the whole polyps correspond to a 3D reconstruction from z-stack series.