Fig. 11

Size differences between the neurocrania of CA and DC larvae. a–f Lateral (a, b), dorsal (c, d) and ventral (e, f) views of dissected alizarin red-/alcian blue-stained neurocranial skeletons of 8 dpf CA (a, c, e) and DC (b, d, f) larvae. Scale bar 0.5 mm. g–j Mean and distribution of 8 dpf CA and DC (n = 5, each) ethmoid–trabeculae length (g), ethmoid cartilage width (h), neurocranial anterior (i) and posterior width (j). k, l Flat-mounted rostral portion of the alcian blue-stained EC in CA (k) and DC (l), dorsal/top, ventral/bottom. m–r Lateral (m, n), dorsal (o, p) and ventral (q, r) views of dissected alizarin red-/alcian blue-stained neurocranial skeletons of 15 dpf CA (m, o, q) and DC (n, p, r) larvae. Scale bar 0.5 mm. s–v Mean and distribution of 15 dpf CA and DC (n = 5, each) ethmoid cartilage length (s) and width (t), and neurocranial anterior (u) and posterior (v) width. ***p < 0.001, Tukey’s HSD test. (w, x) Flat-mounted rostral portion of the alizarin red-/alcian blue-stained EC in 15 dpf CA (w) and DC (x), dorsal/top, ventral/bottom. Bone nomenclature after [64]. B basicapsular fenestra, BP basal plate, BO basiotic, EC ethmoid cartilage, EO epiotic, EOC epioccipital, EPB epiphyseal bar, FR frontal, LE lateral ethmoid, ME mesethmoid, NC notochord, OC otic capsule, PA parietal, PO pterotic, PRO prootic, PS parasphenoid, SO sphenotic, SOC supraoccipital crest, T trabecula, TMA taenia marginalis anterior, TMP taenia marginalis posterior, VO vomer