Anatomy of a choanocyte chamber in A. queenslandica.
a A schematic drawing of a choanocyte chamber consisting of multiple choanocytes with a collar of microvilli and cilium pointing inward. b A confocal section of a choanocyte chamber in A. queenslandica consisting of multiple choanocytes with cilia pointing inward. Nuclei (nu) are stained with DAPI (blue); cilia (ci) are immunofluorescently labeled with an anti-acetylated alpha tubulin antibody as well as CM-DiI, which also label the cell body (red). ci cilium, mi microvilli, nu nucleus. Scale bar 5 μm