Fig. 5
From: The ontogeny of choanocyte chambers during metamorphosis in the demosponge Amphimedon queenslandica
Choanocyte chambers labeled with anti-PH3 labeled nuclei in a 72-hpr juvenile. a, b Confocal sections of single choanocyte chambers. Nuclei are stained with DAPI (blue), and cilia are immunofluorescently labeled with an anti-acetylated alpha tubulin antibody (green), and mitotic nuclei are labeled with anti-phospho-histone H3 antibody (magenta). Note in b that a subset of choanocytes in the chamber is PH3 positive, indicative of mitosis (arrowheads). Scale bar 5 μm