Fig. 6
From: The ontogeny of choanocyte chambers during metamorphosis in the demosponge Amphimedon queenslandica
Proliferating nuclei labeled with EdU during early postlarval development. Nuclei are stained with DAPI (blue) and mitotic nuclei pulse labeled with EdU (green). a, b 24 hpr postlarva pulse labeled with EdU from 18 to 24 hpr. A small number of nuclei are labeled with EdU (arrowheads). c, d 30 hpr postlarva pulse labeled with EdU (from 24 to 30 hpr). An increase in proliferation rate can be observed, with clusters of EdU-positive nuclei evident as well (arrowheads). Scale bars a, c 40 μm; b, d 15 μm