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Fig. 6 | EvoDevo

Fig. 6

From: Neuronal fate specification by the Dbx1 transcription factor is linked to the evolutionary acquisition of a novel functional domain

Fig. 6

In vivo function of Dbx1 from different species. In ovo electroporation experiments using plasmids encoding Dbx homologs from Ciona (ciDbx), Saccoglossus (sDbx), Zebrafish (zDbx1b) as well as Cter-modified versions (ciDbx + Cter and sDbx∆Cter). Spinal cord cryosections were immunostained for Evx1/2 (a) or En1 (c) (red), DAPI (blue) and GFP (green) to visualize the electroporated side. b, d Quantifications of Evx1/2+ (b) or En1+ (d) cells on the electroporated side relative to the control side are indicated. Each dot represents one section, the horizontal bar corresponds to the average for each condition, and statistical significance is color coded (red p < 0.01 using paired Student’s t test; orange p < 0.05; green nonsignificant). Scale bar 50 µm

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