Fig. 4
From: Fibronectin contributes to notochord intercalation in the invertebrate chordate, Ciona intestinalis
Ciona Fibronectin is necessary for intercalation of notochord cells during convergent extension. a–c′′′ Representative micrographs showing lateral projections and accompanying 2-μm orthogonal sections of notochord cells in late tailbud embryos co-transfected as labeled. Red and yellow boxes indicate location of insets. Approximate locations of orthogonal sections (a′–c′′′) are indicated in the associated schematics. Misaligned cells are indicated in orthogonal sections (arrows). Phalloidin (blue). Note that no reduction in notochord cell numbers occurred in these samples. In all 15 U6>FN6sgRNA embryos with bilateral incorporation of the Bra>GFP, 40 notochord cells were present. d Graphical summary of CRISPR/CAS9 data. Data were obtained from three independent trials, n>29/trial. Error bars represent the S.E.M. P values for moderately defective phenotypes in U6>sgRNA (empty) and U6>FN6sgRNA mismatch (controls) versus U6>FN6sgRNA are indicated. P = 0.882 for U6>sgRNA mild versus U6>FNgRNA6 mm mild. Significance was determined using a two-tailed unpaired t test. e Approximate location of gRNA target sequence in FNII domain. Alignment of FN alleles cloned from pooled embryos electroporated with Brac>nls::Cas9::nls and U6>FN6gRNA. Scale bars 25 μm. Embryos oriented anterior to the left