Transcriptomic analysis of maternally provisioned cues for phenotypic plasticity in the annual killifish, Austrofundulus limnaeus

Table 5 Differentially expressed sncRNAs in diapause- and escape-bound embryos of A. limnaeus

Sequence	Bp^a	Exp.^b	Log₂FC^c	Sig.^d	Pheno.^e	15 bp^f	12 bp^f	10 bp^f	8 bp^f	Alignment^g	Orientation^h	Positionⁱ
ACAACGTGTGATACA	15	26.9	5.1	0.008	Escape	1	23	103	183	LOC106517361 (Zinc finger protein 646-like)	Antisense	Intragenic, intronic
TAGTATATAGGACTA	15	46	4.1	0.036	Escape	2	13	60	179	LOC106525548 (neurexin-2-like)/unannotated region	Antisense/sense	Intragenic, intronic/Intergenic
GGCTCTGAATACATTAG	17	5.8	4.5	0.036	Escape	2	126	131	159	LOC106520853 (RNA-binding motif, single-stranded-interacting protein 3)/LOC106533203 (endosialin-like)	Antisense/antisense	Intragenic, intronic/Intragenic, exonic
TCGGAACTCACCCAGTC	17	15.8	4	0.044	Diapause	2	4	6	6	Unannotated region/LOC106512419 (uncharacterized LOC106512419)	Sense/antisense	Intergenic/Intragenic, exonic

^aLength of the sncRNA in base pairs
^bMean counts per million mapped reads across all samples
^cLog₂ fold change
^dStatistical significance (FDR < 0.1)
^eDevelopmental phenotype with the higher abundance of the sncRNA
^fNumber of alignment locations in the A. limnaeus genome for the sequence if the number of bases indicated is used starting at the 5’ end of the sequence
^gThe locations in the A. limnaeus genome where the 15-bp sequence aligned
^hThe orientation of each alignment location for the 15-bp sequence in reference to the A. limnaeus genome annotation
ⁱPosition within the alignment location that the sncRNA aligned to

ISSN: 2041-9139