Fig. 2
From: Establishment of lateral organ asymmetries in the invertebrate chordate, Ciona intestinalis
Ion flux is required for laterally asymmetric heart positioning. a′–a‴ Ventral projections of transgenic Stage 31 larva, and heart founder lineage cells are demarcated by Mesp > H2B:GFP (green), including heart precursors (HP) and atrial siphon muscle precursors (ASM). a′–a‴ Typical asymmetric heart precursor positioning in DMSO controls (a′), larva treated with 5 µM SB431542 (a″) or larva treated with 115 µM omeprazole (a‴). b Schematic illustrating cell shift ratio used for scoring heart precursor positioning, as detailed in the “Methods” section. c Graphical representation of larval heart position data. Error bars represent SEM, and n represents total number of larva scored over three-independent trials. Chi-square test derived P values comparing control and omeprazole-treated larva (p = 2.9E−5) or SB431542-treated samples (p = 0.84). d′–d′′′′ Dorsal view (d′) and lateral sections (d″–d′′′′) of Ciona juveniles displaying a midline heart phenotype following omeprazole treatment. In (d′), the heart is false-colored green and the endostyle is false-colored purple. In lateral sections (d″–d′′′′), heart cell nuclei are labeled by Mesp > H2B:GFP. In all images, anterior is to the left. All scale bars are in micrometers