Fig. 8
From: PaxA, but not PaxC, is required for cnidocyte development in the sea anemone Nematostella vectensis
Co-expression of Mef2IV and PaxA, not PaxC, in developing cnidocytes. a–c Mef2IV mRNA co-localizes with Ncol3 mRNA in only ~25% of the developing cnidocytes. (Cells co-expressing both markers are indicated by arrows, and cells expressing one marker only are indicated by circles in panels a–j). d–f PaxA mRNA co-localizes with Ncol3 mRNA in ~50% of the developing cnidocytes. g–j PaxA mRNA is co-expressed in Mef2IV-expressing cells, but several cells express PaxA without Mef2IV. Co-localization of k, l Mef2IV mRNA and m, n PaxA mRNA with NCOL3 protein. o–r Co-labeling of PaxA and Mef2IV mRNA with α-NCOL3 indicates there are cells expressing all three markers at the same time (arrows), cells expressing PaxA and NCOL3 without Mef2IV (dotted circles), and cells expressing NCOL3 protein without either PaxA or Mef2IV (arrowhead). s–v PaxC is not co-expressed with Ncol3 or w–z PaxA. aa–ad PaxA and SoxB2 are not co-expressed. Insets and main images are 3D reconstructions from confocal z-stacks; high-magnification images are individual optical sections