Fig. 1

Gene expression in H. halys embryos. a In situ hybridization in H. halys germ band embryos using an Hh–en probe. Six stripes were detected. The anterior and posterior parts of the embryo remained covered with yolk in this photograph. b En antibody staining to an embryo slightly older than that shown in (a). Nine stripes were observed. c A blastoderm stage embryo expressing three Hh-eve stripes in the central region of the embryo. d A germ band with two Hh-eve stripes at or near the segment addition zone. (a, b, d) anterior (top) to posterior (bottom); (c) embryo orientated anterior (left), posterior (right)