Fig. 2

Experimental manipulation of Gal8 affects zeugopod, stylopod, and autopod development in the avian wing. Left—wing bud from a 5-day chicken embryo injected with Gal8 protein, grown for a day and then isolated, fixed, and stained for cartilage with Alcian blue. Right—the control, contralateral wing bud (reflected). Experimental treatment results in complete absence of chondrified primordia in the autopod, dysmorphic, aberrantly arranged primordia in the zeugopod, and a weakly stained, poorly developed stylopod. The control limb shows well-developed and stained cartilage primordia in the stylo-, zeugo-, and autopodial regions. Images adapted from [40]