Fig. 7

Relationships among trypsin and ShK proteins in N. vectensis. a Phylogeny of N. vectensis trypsin domains; domain architecture diagrams are shown to the right. Proteins with multiple trypsin domains appear multiple times in the tree; in these cases, the domain represented at each position in the tree is bold. SP—signal peptide, TM—transmembrane domain. Trypsin domains lacking H-57, D-102, or S-195 are designated non-catalytic (see Additional file 4). A tree file with branch support values is available on our Github site (https://github.com/josephryan/2019-Babonis_et_al_trypsins). b Phylogeny of N. vectensis ShK domains from the predicted proteome. Domain architectures for proteins with multiple ShK domains appear multiple times in the tree; in these cases, the domain represented at each position is indicated with an arrowhead. Proteins that have both trypsin and ShK domains are colored green. Colored symbols to the right of the domain architectures are provided to facilitate comparisons of identical proteins in panels a and b. Domains are colored as shown in Fig. 4a